Zhang Ming, Zhou Liang, Xie Ming, Tian Jie
Department of Otolaryngology, Eye Ear Nose Throat Hospital, Fudan University, Shanghai, 200031, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2007 Dec;21(24):1105-8.
To investigate the synergistic cytotoxicity of TRAIL and paclitaxel on laryngeal squamous carcinoma cell line Hep-2.
Hep-2 cells were exposed to various concentrations of TRAIL and paclitaxel respectively or jointly. The inhibition rate was measured by CCK-8 assay. The apoptosis rate and the expressions of DR4, DR5, DcR1 and DcR2 in Hep-2 cells were detected by flow cytometry methods.
Hep-2 cells were resistant to apoptosis induced by TRAIL. Paclitaxel could enhance its sensitivity to TRAIL by up-regulating the expressions of TRAIL death receptors in Hep-2 cells.
The resistance of Hep-2 cell to TRAIL could be overcome by paclitaxel. TRAIL and paclitaxel have synergistic killing effects on Hep-2 cells, so they might have a promising prospect in clinical therapy of laryngeal squamous carcinoma.
