Korenblum E, Sebastián G V, Paiva M M, Coutinho C M L M, Magalhães F C M, Peyton B M, Seldin L
Laboratório de Genética Microbiana, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brazil.
Appl Microbiol Biotechnol. 2008 May;79(1):97-103. doi: 10.1007/s00253-008-1401-x. Epub 2008 Mar 11.
Microbial colonization of petroleum industry systems takes place through the formation of biofilms, and can result in biodeterioration of the metal surfaces. In a previous study, two oil reservoir Bacillus strains (Bacillus licheniformis T6-5 and Bacillus firmus H(2)O-1) were shown to produce antimicrobial substances (AMS) active against different Bacillus strains and a consortium of sulfate-reducing bacteria (SRB) on solid medium. However, neither their ability to form biofilms nor the effect of the AMS on biofilm formation was adequately addressed. Therefore, here, we report that three Bacillus strains (Bacillus pumilus LF4 -- used as an indicator strain, B. licheniformis T6-5, and B. firmus H(2)O-1), and an oil reservoir SRB consortium (T6lab) were grown as biofilms on glass surfaces. The AMS produced by strains T6-5 and H(2)O-1 prevented the formation of B. pumilus LF4 biofilm and also eliminated pre-established LF4 biofilm. In addition, the presence of AMS produced by H(2)O-1 reduced the viability and attachment of the SRB consortium biofilm by an order of magnitude. Our results suggest that the AMS produced by Bacillus strains T6-5 and H(2)O-1 may have a potential for pipeline-cleaning technologies to inhibit biofilm formation and consequently reduce biocorrosion.
石油工业系统中的微生物定殖通过生物膜的形成而发生，并可能导致金属表面的生物劣化。在先前的一项研究中，两种油藏芽孢杆菌菌株（地衣芽孢杆菌T6-5和坚强芽孢杆菌H₂O-1）在固体培养基上显示出对不同芽孢杆菌菌株和硫酸盐还原菌（SRB）联合体具有活性的抗菌物质（AMS）。然而，它们形成生物膜的能力以及AMS对生物膜形成的影响均未得到充分研究。因此，在此我们报告，三种芽孢杆菌菌株（短小芽孢杆菌LF4——用作指示菌株、地衣芽孢杆菌T6-5和坚强芽孢杆菌H₂O-1）以及一个油藏SRB联合体（T6lab）在玻璃表面形成了生物膜。菌株T6-5和H₂O-1产生的AMS阻止了短小芽孢杆菌LF4生物膜的形成，并且还消除了预先形成的LF4生物膜。此外，H₂O-1产生的AMS的存在使SRB联合体生物膜的活力和附着减少了一个数量级。我们的结果表明，地衣芽孢杆菌T6-5和坚强芽孢杆菌H₂O-1产生的AMS可能具有用于管道清洁技术以抑制生物膜形成并因此减少生物腐蚀的潜力。
