Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411 007, India.
Colloids Surf B Biointerfaces. 2010 Nov 1;81(1):242-8. doi: 10.1016/j.colsurfb.2010.07.013. Epub 2010 Jul 13.
Removal of detrimental biofilms from surfaces exposed in the marine environment remains a challenge. A strain of Bacillus pumilus was isolated from the surface of titanium coupons immersed in seawater in the vicinity of Madras Atomic Power Station (MAPS) on the East coast of India. The bacterium formed extensive biofilms when compared to species such as Bacillus licheniformis, Pseudomonas aeruginosa PAO1 and Pseudomonas aureofaciens. A commercially available rhamnolipid was assessed for its ability to inhibit adhesion and disrupt pre-formed B. pumilus biofilms. The planktonic growth of B. pumilus cells was inhibited by concentrations >1.6mM. We studied the effect of various concentrations (0.05-100mM) of the rhamnolipid on adhesion of B. pumilus cells to polystyrene microtitre plates, wherein the effectiveness varied from 46 to 99%. Biofilms of B. pumilus were dislodged efficiently at sub-MIC concentrations, suggesting the role of surfactant activity in removing pre-formed biofilms. Scanning electron microscopy (SEM) confirmed the removal of biofilm-matrix components and disruption of biofilms by treatment with the rhamnolipid. The results suggest the possible use of rhamnolipids as efficient anti-adhesive and biofilm-disrupting agents with potential applications in controlling biofilms on surfaces.
从暴露于海洋环境中的表面去除有害生物膜仍然是一个挑战。从印度东海岸马德拉斯原子发电站（MAPS）附近海水中浸泡的钛片表面分离出一株解淀粉芽孢杆菌。与地衣芽孢杆菌、铜绿假单胞菌 PAO1 和金黄色葡萄球菌等物种相比，该细菌形成了广泛的生物膜。评估了一种市售的鼠李糖脂，以研究其抑制粘附和破坏预先形成的解淀粉芽孢杆菌生物膜的能力。解淀粉芽孢杆菌细胞的浮游生长被浓度>1.6mM 的物质抑制。我们研究了不同浓度（0.05-100mM）的鼠李糖脂对解淀粉芽孢杆菌细胞粘附到聚苯乙烯微量滴定板的影响，其有效性从 46%到 99%不等。在亚 MIC 浓度下，生物膜被有效地洗脱，这表明表面活性剂活性在去除预先形成的生物膜中起作用。扫描电子显微镜（SEM）证实了生物膜基质成分的去除和生物膜的破坏通过鼠李糖脂处理。结果表明，鼠李糖脂可能作为有效的抗粘附和生物膜破坏剂，具有在控制表面生物膜方面的潜在应用。
