Izquierdo E, Medina M, Ennahar S, Marchioni E, Sanz Y
Instituto de Agroquímica y Tecnología de Alimentos, 46015, Burjassot-Valencia, Spain.
Curr Microbiol. 2008 Jun;56(6):613-8. doi: 10.1007/s00284-008-9135-7. Epub 2008 Mar 11.
Eight Bifidobacterium longum strains, including reported probiotic strains (commercial and noncommercial), collection strains, and laboratory isolates, were investigated for their ability to adhere to mucin as well as their ability to tolerate acid and bile. Strains could be discriminated based on their sensitivity at pH values of 2.0 to 2.5 and bile concentrations of 0.5% to 2.0%. B. longum NCC 2705, a strain known for its probiotic properties, showed the highest resistance to gastrointestinal conditions, whereas the commercial probiotic strains B. longum BB 536 and SP 07/3 were the least resistant. In parallel, the human isolate B. longum BIF 53 showed the highest adhesion to mucin, whereas the commercial probiotic strains B. longum W 11, BB 536, and SP 07/3 were the least adhesive. The bacterial adhesion to mucin of strains B. longum NCC 2705 and BIF 53 could be reduced by lysozyme, indicating that cell-wall components are involved in the adhesion process. These results showed that there is no obvious link between adhesion and resistance to gastrointestinal conditions and the probiotic status of the studied strains. This calls for a definition of conditions for in vitro tests that better predict the in vivo functionality of probiotic strains.
对8株长双歧杆菌菌株进行了研究，这些菌株包括已报道的益生菌菌株（商业菌株和非商业菌株）、保藏菌株和实验室分离株，检测它们黏附黏蛋白的能力以及耐受酸和胆汁的能力。可根据菌株在pH值为2.0至2.5以及胆汁浓度为0.5%至2.0%时的敏感性对其进行区分。具有益生菌特性的长双歧杆菌NCC 2705对胃肠道条件的耐受性最高，而商业益生菌菌株长双歧杆菌BB 536和SP 07/3的耐受性最低。同时，人源分离株长双歧杆菌BIF 53对黏蛋白的黏附性最高，而商业益生菌菌株长双歧杆菌W 11、BB 536和SP 07/3的黏附性最低。溶菌酶可降低长双歧杆菌NCC 2705和BIF 53菌株对黏蛋白的细菌黏附，这表明细胞壁成分参与了黏附过程。这些结果表明，在所研究菌株的黏附性、对胃肠道条件的耐受性与益生菌状态之间没有明显联系。这就需要定义体外试验条件，以便更好地预测益生菌菌株的体内功能。
