Guan Chengran, Chen Xuan, Jiang Xinrong, Zhao Ruifeng, Yuan Yuan, Chen Dawei, Zhang ChenChen, Lu Maolin, Lu Zhen, Gu Ruixia
Key Lab of Dairy Biotechnology and Safety Control, College of Food Science and Engineering, Yangzhou University Yangzhou Jiangsu 225127 China
Bloomage Biotechnology Corporation Limited Ji'nan Shandong 250000 China.
RSC Adv. 2020 Jun 25;10(41):24234-24240. doi: 10.1039/d0ra03517c. eCollection 2020 Jun 24.
Six lactic acid bacteria (LAB), isolated from the intestinal tract of the longevous population, were prominent for their strong bacteriostatic ability. In this study, the adhesion properties of the six strains were determined to explore their potential to be used as probiotics. The hydrophobicity and aggregation activity were firstly detected and were varied from 14.83% to 57.3% and 12.7% to 31%, respectively. Moreover, the adhesion activity to the intestinal crypt cells (IEC-6 cells) was proved to be varied from 5.4 to 21.7 bacteria numbers per cell. Furthermore, all the tested LAB samples could inhibit 3 Gram-positive and 3 Gram-negative indicator microorganisms to adhere to the IEC-6 cells. Meanwhile every sample was inclined to exclude rather than displace or compete to inhibit the indicator microorganisms to adhere to IEC-6 cells. Afterwards, the adhesion activities of the LAB were demonstrated to be highly affected by the surface proteins considering the treatments of heat, pepsin, trypsin and NaIO. The surface proteins (8-14 kDa) of every sample were isolated and proved to be helpful to regain more than 30% of the adhesion activity for the corresponding samples. This study will be beneficial to examine the characteristics of these strains especially g9 when used as probiotics in dairy food products.
从长寿人群肠道中分离出的6株乳酸菌（LAB），因其强大的抑菌能力而备受关注。在本研究中，测定了这6株菌的黏附特性，以探索它们作为益生菌的潜力。首先检测了疏水性和聚集活性，其范围分别为14.83%至57.3%和12.7%至31%。此外，对肠道隐窝细胞（IEC-6细胞）的黏附活性经证实为每个细胞5.4至21.7个细菌数。此外，所有测试的LAB样品均可抑制3种革兰氏阳性和3种革兰氏阴性指示微生物黏附至IEC-6细胞。同时，每个样品倾向于排斥而非取代或竞争抑制指示微生物黏附至IEC-6细胞。之后，考虑到热、胃蛋白酶、胰蛋白酶和NaIO处理，LAB的黏附活性被证明受表面蛋白的高度影响。分离出每个样品的表面蛋白（8 - 14 kDa），并证明其有助于相应样品恢复超过30%的黏附活性。本研究将有助于考察这些菌株，尤其是g9菌株在乳制品中用作益生菌时的特性。
