Bradley M, Alexander L, Sanchez-Martin R M
Chemical Biology Section, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, UK.
J Fluoresc. 2008 May-Jul;18(3-4):733-9. doi: 10.1007/s10895-008-0334-1. Epub 2008 Mar 11.
Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA. These biotin-streptavidin microsphere conjugates were then successfully delivered into cells. The application of the streptavidin-biotin technology to these microspheres allows the effective delivery of any biotinylated material into intact mammalian cells, without the need for delicate procedures such as micro-injection.
氨基功能化的交联聚苯乙烯微球通过共价键负载链霉亲和素，荧光标记的生物素和生物素化标记的DNA与链霉亲和素偶联。然后，这些生物素 - 链霉亲和素微球缀合物成功地递送至细胞中。将链霉亲和素 - 生物素技术应用于这些微球，可将任何生物素化材料有效地递送至完整的哺乳动物细胞中，而无需微注射等精细操作。
