用于活细胞中离子传感与成像的生物定位嵌入封装纳米级探针（PEBBLEs）。

Nanoscale probes encapsulated by biologically localized embedding (PEBBLEs) for ion sensing and imaging in live cells.

作者信息

Buck Sarah M, Xu Hao, Brasuel Murphy, Philbert Martin A, Kopelman Raoul

机构信息

Department of Chemistry, The University of Michigan, Ann Arbor, MI 48109-1055, USA.

出版信息

Talanta. 2004 May 10;63(1):41-59. doi: 10.1016/j.talanta.2003.12.048.

DOI:10.1016/j.talanta.2003.12.048

PMID:18969403

Abstract

This review discusses the development and recent advances of probes encapsulated by biologically localized embedding (PEBBLEs), and in particular the application of PEBBLEs as ion sensors. PEBBLEs allow for minimally intrusive sensing of ions in cellular environments due to their small size (20 to 600nm in diameter) and protect the sensing elements (i.e. fluorescent dyes) by encapsulating them within an inert matrix. The selectivity and sensitivity of these nanosensors are comparable to those of macroscopic ion selective optodes, and electrodes, while the response time and absolute detection limit are significantly better. This paper discusses the principles guiding PEBBLE design including synthesis, characterization, diversification, the advantages and limitations of the sensors, cellular applications and future directions of PEBBLE research.

摘要

本综述讨论了生物定位嵌入（PEBBLEs）封装探针的发展及近期进展，尤其着重于PEBBLEs作为离子传感器的应用。由于其尺寸小（直径20至600纳米），PEBBLEs能够在细胞环境中以最小的侵入性对离子进行传感，并通过将传感元件（即荧光染料）封装在惰性基质中来保护它们。这些纳米传感器的选择性和灵敏度与宏观离子选择性光极及电极相当，而响应时间和绝对检测限则显著更优。本文讨论了指导PEBBLE设计的原理，包括合成、表征、多样化、传感器的优缺点、细胞应用以及PEBBLE研究的未来方向。