Rencüzoğullari Eyyüp, Parlak Sebnem, Ila Hasan Basri
Department of Biology, Faculty of Science and Letters, Cukurova University, Adana, Turkey.
Drug Chem Toxicol. 2008;31(2):263-74. doi: 10.1080/01480540701873285.
The aim of this study was to determine the possible genotoxic effects of biphenyl (E230), which is used as an antimicrobial agent in food by using sister chromatid exchanges (SCEs), chromosome aberrations (CAs), and micronucleus (MN) tests in human peripheral lymphocytes. The human peripheral lymphocytes were treated with four concentrations of biphenyl (10, 30, 50, and 70 microg/mL) for 24- and 48-h treatment periods. In the present study, biphenyl significantly increased the frequency of SCEs, CAs, and the frequency of MN when compared with both untreated control and solvent (dimethyl sulfoxide) control. The inductions of these abnormalities were in a dose-dependent manner. Biphenyl was capable to induce the structural CAs instead of numerical CAs. Biphenyl also showed a cytotoxic effect by decreasing the replication index at the highest two concentrations for 48 h and nuclear division index at the highest two concentrations for the 24- and 48-h treatment periods. However, biphenyl did not affect the mitotic index (MI).
本研究的目的是通过在人外周血淋巴细胞中进行姐妹染色单体交换（SCE）、染色体畸变（CA）和微核（MN）试验，确定用作食品抗菌剂的联苯（E230）可能的遗传毒性作用。将人外周血淋巴细胞用四种浓度的联苯（10、30、50和70微克/毫升）处理24小时和48小时。在本研究中，与未处理的对照和溶剂（二甲基亚砜）对照相比，联苯显著增加了SCE、CA的频率以及MN的频率。这些异常的诱导呈剂量依赖性。联苯能够诱导结构性CA而非数目性CA。联苯在最高的两个浓度下处理48小时时通过降低复制指数，以及在最高的两个浓度下处理24小时和48小时时通过降低核分裂指数，还表现出细胞毒性作用。然而，联苯不影响有丝分裂指数（MI）。
