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2
Evaluation of angiogenesis with the expression of VEGF and CD34 in human non-small cell lung cancer.通过检测VEGF和CD34表达评估人非小细胞肺癌中的血管生成情况。
J Exp Clin Cancer Res. 2007 Sep;26(3):375-8.
3
A hypoxia-independent up-regulation of hypoxia-inducible factor-1 by AKT contributes to angiogenesis in human gastric cancer.AKT对缺氧诱导因子-1的非缺氧依赖性上调促进了人胃癌的血管生成。
Carcinogenesis. 2008 Jan;29(1):44-51. doi: 10.1093/carcin/bgm232. Epub 2007 Nov 4.
4
The biological role of HGF-MET axis in tumor growth and development of metastasis.肝细胞生长因子-间质-上皮转化因子轴在肿瘤生长和转移发展中的生物学作用。
Front Biosci. 2008 Jan 1;13:1271-80. doi: 10.2741/2760.
5
Prognostic significance of platelet-derived growth factor-BB expression in human esophageal squamous cell carcinomas.血小板衍生生长因子-BB在人食管鳞状细胞癌中的表达的预后意义
Anticancer Res. 2007 Jul-Aug;27(4B):2409-14.
6
Development of strategies for conditional RNA interference.条件性RNA干扰策略的开发。
J Gene Med. 2007 Apr;9(4):287-98. doi: 10.1002/jgm.1018.
7
Using attenuated Salmonella typhi as tumor targeting vector for MDR1 siRNA delivery.使用减毒伤寒沙门氏菌作为MDR1 siRNA递送的肿瘤靶向载体。
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Cytokine. 2006 Dec;36(5-6):291-5. doi: 10.1016/j.cyto.2007.02.008. Epub 2007 Mar 19.
9
Effect of vascular normalization by antiangiogenic therapy on interstitial hypertension, peritumor edema, and lymphatic metastasis: insights from a mathematical model.抗血管生成疗法实现血管正常化对间质高血压、肿瘤周围水肿和淋巴转移的影响：来自数学模型的见解
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血管生成素-1靶向RNA干扰抑制食管癌的血管生成和肿瘤生长。

Angiopoietin-1 targeted RNA interference suppresses angiogenesis and tumor growth of esophageal cancer.

作者信息

Liu Xiao-Hong, Bai Chen-Guang, Yuan Yang, Gong De-Jun, Huang Sheng-Dong

机构信息

Institute of Thoracardiac Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.

出版信息

World J Gastroenterol. 2008 Mar 14;14(10):1575-81. doi: 10.3748/wjg.14.1575.

DOI:10.3748/wjg.14.1575
PMID:18330951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2693755/
Abstract

AIM

To determine the inhibitory effect of the adenovirus-based angiopoietin-1 (Ang-1) targeted small interfering RNA expression system (Ad/Ang-1si) on the expression of the Ang-1 gene, cell growth and apoptosis in human esophageal cancer cell line Eca109.

METHODS

siRNA-expressing adenovirus targeting Ang-1 gene was constructed using the Ad Easy System. Cultured Eca109 cells were transfected with Ad/Ang-1si (Eca109/Ang-1si), and Ad/si was used to infect Eca109 cells as control (Eca109/si). Ang-1 gene expression and concentration was determined with RT-PCR and ELISA, respectively. Human umbilical vein endothelial cell (HUVEC) migration and proliferation were analyzed. After s.c. injection into athymic nu/nu mice, the tumor growth, vessel density and apoptosis of each group was also determined.

RESULTS

HUVEC migration induced by conditioned medium from Ang-1si-transfected Eca109 cells was significantly less than that induced by conditioned medium from Eca109 cells and control adenovirus-transfected Eca109 cells. Furthermore, after s.c. injection into athymic nu/nu mice, the tumor growth and cell apoptosis of Ad/Ang-1si -expressing Eca109 cells was significantly lower than that of parental or control adenovirus-transfected cells. Vessel density assessed by CD31 immunohistochemical analysis and Ang-1 expression by RT-PCR were also decreased.

CONCLUSION

The targeting Ang-1 may provide a therapeutic option for esophageal cancer.

摘要

目的

确定基于腺病毒的血管生成素-1（Ang-1）靶向小干扰RNA表达系统（Ad/Ang-1si）对人食管癌Eca109细胞系中Ang-1基因表达、细胞生长及凋亡的抑制作用。

方法

利用Ad Easy系统构建靶向Ang-1基因的表达小干扰RNA的腺病毒。用Ad/Ang-1si转染培养的Eca109细胞（Eca109/Ang-1si），并用Ad/si感染Eca109细胞作为对照（Eca109/si）。分别用逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）法测定Ang-1基因表达及浓度。分析人脐静脉内皮细胞（HUVEC）的迁移和增殖情况。将细胞皮下注射到无胸腺裸鼠体内后，还测定了每组的肿瘤生长、血管密度及凋亡情况。

结果

由Ang-1si转染的Eca109细胞的条件培养基诱导的HUVEC迁移明显少于Eca109细胞及对照腺病毒转染的Eca109细胞的条件培养基诱导的迁移。此外，皮下注射到无胸腺裸鼠体内后，表达Ad/Ang-1si的Eca109细胞的肿瘤生长及细胞凋亡明显低于亲本细胞或对照腺病毒转染的细胞。通过CD31免疫组织化学分析评估的血管密度及RT-PCR检测的Ang-1表达也降低。

结论

靶向Ang-1可能为食管癌提供一种治疗选择。