Huang Anderson Hsien-Cheng, Chen Yuk-Kwan, Lin Lin-Min, Shieh Tien-Yue, Chan Anthony Wing-Sang
Grace Dental Clinic, School of Dentistry, Kaosiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
J Oral Pathol Med. 2008 Oct;37(9):571-4. doi: 10.1111/j.1600-0714.2008.00654.x. Epub 2008 Mar 6.
Dental pulp stem cells (DPSCs) were primarily derived from the pulp tissues of primary incisors and permanent third molar teeth, whereas no report to our knowledge has yet been documented on deriving DPSCs from the other tooth types. The aim of this study is to present a novel approach of harvesting stem cells from a supernumerary tooth (a mesiodens).
The pulp tissues from a mesiodens of a 20-year-old healthy male patient and the left lower deciduous canine of a healthy 10-year-old boy (the positive control) were extracted and cultured for DPSCs, which were examined with stem cells (Oct-4, Nanog and Rex-1) and differentiation (Osteonectin and Nestin) markers. Furthermore, DPSCs were directionally differentiated to osteogenic and adipogenic cell lineages.
Dental pulp stem cells derived from the mesiodens were capable of differentiating into adipogenic and osteogenic lineages. The mesioden's DPSCs also expressed stem cell and differentiation markers, which suggested their stem cell origin and differentiation capability. All the aforementioned results for the mesiodens were consistent with those of the DPSCs derived from the positive control.
We have demonstrated the feasibility of deriving DPSCs from a usually discarded tissue such as a supernumerary tooth.
牙髓干细胞（DPSCs）主要来源于乳切牙和恒第三磨牙的牙髓组织，然而据我们所知，尚无从其他牙齿类型中获取DPSCs的相关报道。本研究的目的是提出一种从多生牙（正中牙）中获取干细胞的新方法。
从一名20岁健康男性患者的正中牙以及一名10岁健康男孩的左下乳尖牙（阳性对照）中提取牙髓组织，培养牙髓干细胞，并使用干细胞标记物（Oct-4、Nanog和Rex-1）和分化标记物（骨连接蛋白和巢蛋白）进行检测。此外，将牙髓干细胞定向分化为成骨和成脂细胞系。
源自正中牙的牙髓干细胞能够分化为成脂和成骨细胞系。正中牙的牙髓干细胞也表达干细胞和分化标记物，这表明了它们的干细胞来源和分化能力。正中牙的所有上述结果与源自阳性对照的牙髓干细胞的结果一致。
我们已经证明了从诸如多生牙这种通常被丢弃的组织中获取牙髓干细胞的可行性。
