Li Hong-Lei, Chen Xiao-Guang, Zhang Fu-Chun, Ma Ji, Xu Cun-Shuan
College of Life Science and Technology, Xinjiang University, Urmuqi 830046, China.
Yi Chuan. 2008 Mar;30(3):333-40. doi: 10.3724/sp.j.1005.2008.00333.
It has been well known that the extracellular matrix (ECM) plays an important role in cell polarization, cell adhesion, cell proliferation, morphogenesis and differentiation. For the sake of the further in-depth investigation of the changes and actions of ECM in liver regeneration (LR) at gene transcriptional level, the ECM-associated genes were obtained by databases searching and literature retrieving, subsequently their expression profiles in rat regenerating liver were detected using Rat Genome 230 2.0 array, then LR-associated genes were identified based on comparison of the gene expression difference between sham operation (SO) group and partial hepatectomy (PH) group. A total of 97 genes were verified to be LR-associated. The initially and totally expressed number of these genes occurring in initial phase of LR, G0/G1 transition, cell proliferation, cell differentiation and structure-functional reconstruction were 49, 19, 43, 5 and 84, 51, 369, 144, respectively, illustrating that expression of the ECM-associated genes were initiated mainly in the early phase, working in differ-ent phases. Their expression similarity was classified into 5 groups including only up-, predominantly up-, only down-, predominantly down-, and equal in up-regulated and down-regulated, involving 38, 21, 21, 10 and 7 genes, respectively; the number of up-regulated expressed genes and down-regulated expressed ones was 411 and 186; their expression patterns were categorized into 24 types, showing that the physiological and biochemical activities in LR were characterized by phase, diversity and intricacy. According to expression profiles and expression patterns of the ECM-associated genes in LR, it was confirmed that the levels of the below-listed genes in expression increased at the corresponding phases of LR, including fibronectin-associated genes at early phase in LR, and collagen-associated genes at middle phase.
众所周知，细胞外基质（ECM）在细胞极化、细胞黏附、细胞增殖、形态发生和分化中发挥着重要作用。为了在基因转录水平上进一步深入研究ECM在肝再生（LR）中的变化和作用，通过数据库搜索和文献检索获得了ECM相关基因，随后使用大鼠基因组230 2.0芯片检测它们在大鼠再生肝中的表达谱，然后基于假手术（SO）组和部分肝切除术（PH）组之间基因表达差异的比较鉴定出LR相关基因。总共97个基因被证实与LR相关。这些基因在LR初始阶段、G0/G1期转换、细胞增殖、细胞分化以及结构功能重建阶段的初始表达数量和总表达数量分别为49、19、43、5以及84、51、369、144，这表明ECM相关基因的表达主要在早期启动，并在不同阶段发挥作用。它们的表达相似性被分为5组，包括仅上调、主要上调、仅下调、主要下调以及上调和下调相等，分别涉及38、21、21、10和7个基因；上调表达基因和下调表达基因的数量分别为411和186；它们的表达模式被分为24种类型，表明LR中的生理生化活动具有阶段性、多样性和复杂性。根据LR中ECM相关基因的表达谱和表达模式，证实了以下列出的基因在LR相应阶段的表达水平升高，包括LR早期的纤连蛋白相关基因以及中期的胶原蛋白相关基因。
