大鼠肝脏再生过程中凝血反应相关基因的表达模式及作用分析
Expression patterns and action analysis of genes associated with blood coagulation responses during rat liver regeneration.
作者信息
Zhao Li-Feng, Zhang Wei-Min, Xu Cun-Shuan
机构信息
Faculty of Life Science and Technology, Ocean University of China, China.
出版信息
World J Gastroenterol. 2006 Nov 14;12(42):6842-9. doi: 10.3748/wjg.v12.i42.6842.
AIM
To study the blood coagulation response after partial hepatectomy (PH) at transcriptional level.
METHODS
After PH of rats, the associated genes with blood coagulation were obtained through reference to the databases, and the gene expression changes in rat regenerating liver were analyzed by the Rat Genome 230 2.0 array.
RESULTS
It was found that 107 genes were associated with liver regeneration. The initially and totally expressing gene numbers occurring in initiation phase of liver regeneration (0.5-4 h after PH), G0/G1 transition (4-6 h after PH), cell proliferation (6-66 h after PH), cell differentiation and structure-function reconstruction (66-168 h after PH) were 44, 11, 58, 7 and 44, 33, 100, 71 respectively, showing that the associated genes were mainly triggered in the forepart and prophase, and worked at different phases. According to their expression similarity, these genes were classified into 5 groups: only up-, predominantly up-, only down-, predominantly down-, up- and down-regulation, involving 44, 8, 36, 13 and 6 genes, respectively, and the total times of their up- and down-regulation expression were 342 and 253, respectively, demonstrating that the number of the up-regulated genes was more than that of the down-regulated genes. Their time relevance was classified into 15 groups, showing that the cellular physiological and biochemical activities were staggered during liver regeneration. According to gene expression patterns, they were classified into 29 types, suggesting that their protein activities were diverse and complex during liver regeneration.
CONCLUSION
The blood coagulation response is enhanced mainly in the forepart, prophase and anaphase of liver regeneration, in which the response in the forepart, prophase of liver regeneration can prevent the bleeding caused by partial hepatectomy, whereas that in the anaphase contributes to the structure-function reorganization of regenerating liver. In the process, 107 genes associated with liver regeneration play an important role.
目的
在转录水平研究部分肝切除术后的凝血反应。
方法
大鼠部分肝切除术后，通过查阅数据库获取与凝血相关的基因，并用大鼠基因组230 2.0芯片分析大鼠再生肝中的基因表达变化。
结果
发现107个基因与肝再生相关。肝再生起始期（部分肝切除后0.5 - 4小时）、G0/G1期转换（部分肝切除后4 - 6小时）、细胞增殖期（部分肝切除后6 - 66小时）、细胞分化及结构 - 功能重建期（部分肝切除后66 - 168小时）出现的初始表达基因数和总表达基因数分别为44、11、58、7和44、33、100、71，表明相关基因主要在前期和早期被触发，并在不同阶段发挥作用。根据它们的表达相似性，这些基因被分为5组：仅上调、主要上调、仅下调、主要下调、上调和下调，分别涉及44、8、36、13和6个基因，其上调和下调表达的总次数分别为342和253，表明上调基因的数量多于下调基因。它们的时间相关性被分为15组，表明肝再生过程中细胞的生理生化活动是交错进行的。根据基因表达模式，它们被分为29种类型，表明肝再生过程中它们的蛋白活性多样且复杂。
结论
凝血反应主要在肝再生的前期、早期和后期增强，其中肝再生前期、早期的反应可防止部分肝切除引起的出血，而后期的反应有助于再生肝的结构 - 功能重组。在此过程中，107个与肝再生相关的基因发挥了重要作用。
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