Brophy C M, Ito R K, Quist W C, Rosenblatt M S, Contreras M, Tsoukas A, LoGerfo F W
New England Deaconess Hospital, Harvard Surgical Service, Boston, Massachusetts 02215.
J Biomed Mater Res. 1991 Aug;25(8):1031-8. doi: 10.1002/jbm.820250809.
Various models have been proposed to examine blood-prosthetic materials interactions in terms of the effect of the prosthetic material on platelet structure and function, blood coagulation and fibrinolysis, and tissue infiltrates (cellular or acellular). In addition, these models have been used to examine the change in the graft surface over time. Particular difficulties in examining graft-materials interactions include species differences, short residence time for blood-materials interactions with commonly employed short grafts, and length of study limitations with ex vivo shunts. In this paper we report a canine, carotid-aorta subcutaneous prosthetic graft model. The specific advantages of this model are the length of the graft, which allows prolonged contact of blood with the prosthetic surface; the subcutaneous location of the graft, which allows repeated sampling of blood along the graft; and the healing characteristics of canine grafts. We selected the canine model because the healing characteristics are morphologically similar to those in humans in that endothelialization of the prosthetic surface is limited. Other models, such as the pig, are favored for use when examining blood coagulation, platelet, or fibrinolytic studies; however, these models can fully endothelialize prosthetic surfaces.
已经提出了各种模型，用于从人工材料对血小板结构和功能、血液凝固和纤维蛋白溶解以及组织浸润（细胞或无细胞）的影响方面来研究血液与人工材料的相互作用。此外，这些模型还被用于研究移植物表面随时间的变化。研究移植物与材料相互作用时的特殊困难包括物种差异、常用短移植物中血液与材料相互作用的停留时间短，以及体外分流研究的时间长度限制。在本文中，我们报告了一种犬类颈动脉 - 主动脉皮下人工移植物模型。该模型的具体优点包括移植物的长度，这使得血液能够与人工表面长时间接触；移植物的皮下位置，这允许沿移植物重复采集血液样本；以及犬类移植物的愈合特性。我们选择犬类模型是因为其愈合特性在形态上与人类相似，即人工表面的内皮化受到限制。其他模型，如猪模型，在研究血液凝固、血小板或纤维蛋白溶解时更受青睐；然而，这些模型的人工表面能够完全内皮化。
