Eroğlu Ela, Eroğlu Inci, Gündüz Ufuk, Yücel Meral
Department of Chemical Engineering, Middle East Technical University, 06531 Ankara, Turkey.
Bioresour Technol. 2008 Oct;99(15):6799-808. doi: 10.1016/j.biortech.2008.01.076. Epub 2008 Mar 10.
The aim of this paper was to gain further insight into the effect of the clay pretreatment process on photofermentative hydrogen production. This two-stage process involved a clay pretreatment step followed by photofermentation which was performed under anaerobic conditions with the illumination by Tungsten lamps. Rhodobacter sphaeroides O.U.001 was used for photofermentation. Higher amounts of color (65%), total phenol (81%) and chemical oxygen demand (31%) removal efficiencies were achieved after clay pretreatment process. During photofermentative hydrogen production with the effluent of clay pretreatment process, the main organic compounds resulting higher hydrogen production rates were found to be acetic, lactic, propionic, and butyric acids. Compared to photofermentation using raw olive mill wastewater ( 16LH2/LOMW), the amount of photofermentative hydrogen production was doubled by using the effluent of the clay pretreatment process (31.5LH2/LOMW). The reasons for the improvement of hydrogen production by clay treatment can be attributed to the high removal of the hardly biodegradable compounds such as phenols; minor removal of organic acids, sugars and amino acids that are known to enhance photofermentative hydrogen production; and the color depletion of raw OMW which might cause a shadowing effect on the photosynthetic bacteria.
本文的目的是进一步深入了解黏土预处理过程对光发酵产氢的影响。这个两阶段过程包括一个黏土预处理步骤，随后是在厌氧条件下用钨灯照明进行的光发酵。球形红细菌O.U.001用于光发酵。黏土预处理过程后，颜色（65%）、总酚（81%）和化学需氧量（31%）的去除效率更高。在用黏土预处理过程的出水进行光发酵产氢期间，发现导致更高产氢率的主要有机化合物是乙酸、乳酸、丙酸和丁酸。与使用生橄榄果渣废水进行光发酵（16LH2/LOMW）相比，使用黏土预处理过程的出水时光发酵产氢量增加了一倍（31.5LH2/LOMW）。黏土处理提高产氢的原因可归因于对酚类等难生物降解化合物的高去除率；对已知可增强光发酵产氢的有机酸、糖和氨基酸的少量去除；以及生橄榄果渣废水颜色的减少，这可能会对光合细菌产生遮蔽效应。
