Huang Sha, Deng Tianzheng, Wang Yijuan, Deng Zhihong, He Lisheng, Liu Shouxin, Yang Jiaji, Jin Yan
Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, Xi'an, China.
Acta Biomater. 2008 Jul;4(4):1057-66. doi: 10.1016/j.actbio.2008.02.007. Epub 2008 Mar 4.
The transplantation of cell-polymer constructs has been developed as a novel approach to curing tissue defects. However, a number of methodological problems remain to be solved, including the loss of a proper cellular milieu, the relatively long period of culture time and the complexity of the application. The aim of the present article is to evaluate the feasibility of porous gelatin-based implantable particles as a novel strategy for delivery of cultured cells and bioactive molecules to correct dermal defects. For this purpose, implantable porous gelatin particles (100-230 microm) encapsulating proliferative growth factors were prepared and characterized, and their influence on fibroblasts was assessed. In vivo examinations were undertaken to observe guided dermal tissue regeneration after the transplantation of the implantable particles. Our results indicate the feasibility of transplanting multifunctional implantable particles as a culture substrate, as a protein transplantation vehicle or as a biodegradable implant for skin regeneration, thus giving an indication of the possible applications in tissue engineering.
细胞-聚合物构建体的移植已发展成为一种治疗组织缺损的新方法。然而，仍有许多方法学问题有待解决，包括合适细胞微环境的丧失、相对较长的培养时间以及应用的复杂性。本文的目的是评估基于多孔明胶的可植入颗粒作为一种将培养细胞和生物活性分子递送至真皮缺损部位以进行修复的新策略的可行性。为此，制备并表征了包裹增殖生长因子的可植入多孔明胶颗粒（100-230微米），并评估了它们对成纤维细胞的影响。进行了体内检查以观察可植入颗粒移植后引导的真皮组织再生。我们的结果表明，移植多功能可植入颗粒作为培养底物、蛋白质移植载体或用于皮肤再生的可生物降解植入物是可行的，从而为组织工程中的可能应用提供了线索。
