Giambanco I, Pula G, Ceccarelli P, Bianchi R, Donato R
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
J Histochem Cytochem. 1991 Sep;39(9):1189-98. doi: 10.1177/39.9.1833446.
We investigated the cellular distribution of annexin V (CaBP33) in rat tissues by immunohistochemistry. Several cell types were shown to express the protein. Glial cells in the cerebellum and in the optic nerve, the corneal epithelium, the posterior epithelium in the iris, chondrocytes, skeletal muscle cells and cardiomyocytes, the capillary endothelial cells in many organs, the muscularis mucosae and the muscular layer in the intestinal tract, hepatocytes, Müller cells in the retina, the lens fibers, Sertoli and Leydig cells in the testis, and smooth muscle cells in the epididymis and bronchi displayed intense immunostaining. In the adrenal gland, only the cortex showed immunoreaction product. In the kidney, no apparent staining of renal cells was observed, whereas endothelial cells of peritubular capillaries were stained. In the heart, annexin V was found associated exclusively with the sarcolemma and intercalated discs, as opposed to the diffuse distribution of the protein in skeletal muscle cells. In the spleen, only reticular elements in the white pulp and endothelial cells in the red pulp appeared to be immunostained. The present data complement the biochemical work thus far done on annexin V and suggest that the protein is neither restricted to secretory cells nor exclusively related to exocytotic events in secretory cells.
我们通过免疫组织化学方法研究了膜联蛋白V（CaBP33）在大鼠组织中的细胞分布。结果显示，几种细胞类型表达该蛋白。小脑和视神经中的神经胶质细胞、角膜上皮、虹膜后上皮、软骨细胞、骨骼肌细胞和心肌细胞、许多器官中的毛细血管内皮细胞、肠道中的黏膜肌层和肌层、肝细胞、视网膜中的 Müller 细胞、晶状体纤维、睾丸中的支持细胞和间质细胞，以及附睾和支气管中的平滑肌细胞均呈现强烈免疫染色。在肾上腺中，只有皮质显示有免疫反应产物。在肾脏中，未观察到肾细胞有明显染色，而肾小管周围毛细血管的内皮细胞被染色。在心脏中，膜联蛋白V仅与肌膜和闰盘相关联，这与该蛋白在骨骼肌细胞中的弥漫分布不同。在脾脏中，只有白髓中的网状成分和红髓中的内皮细胞呈现免疫染色。目前的数据补充了迄今为止关于膜联蛋白V的生化研究工作，并表明该蛋白既不限于分泌细胞，也不专门与分泌细胞中的胞吐事件相关。
