Feinberg J M, Rainteau D P, Kaetzel M A, Dacheux J L, Dedman J R, Weinman S J
Departement de Biochimie, UFR Biomédicale des Saintes-Pères, Paris, France.
J Histochem Cytochem. 1991 Jul;39(7):955-63. doi: 10.1177/39.7.1830893.
We used antibodies that specifically bind annexins on Western blots to determine the distribution and abundance of these proteins in ram spermatids and sperm by immunogold electron microscopy. Annexins I and II were found essentially within the entire acrosome of spermatids. During epididymal maturation, they concentrated in the postacrosomal region or the acrosomal equatorial segment, respectively. They were also present in sperm flagellum, on the surface of the coarse fibers and fibrous sheath. These findings show that during ram germ cell maturation, annexins I and II are exported from the spermatid acrosome towards structurally and functionally defined parts of the sperm. Annexins III, IV, and V were not found in ram germ cells. Annexin VI was isolated from testis and sperm. In spermatids, it was found to be associated with endoplasmic reticulum and the mitochondria but was absent from the acrosome. In sperm, it was confined to the flagellum, the mitochondria, and on the coarse fibers and fibrous sheath. The presence of three annexins, in addition to calmodulin, in functional areas may indicate differential ways for sperm to control and regulate events that are known to be calcium dependent, such as flagellar motility, acrosome reaction, and fertilization.
我们使用在蛋白质免疫印迹法中能特异性结合膜联蛋白的抗体，通过免疫金电子显微镜来确定这些蛋白质在公羊精子细胞和精子中的分布及丰度。膜联蛋白I和II主要存在于精子细胞的整个顶体内。在附睾成熟过程中，它们分别集中在顶体后区域或顶体赤道段。它们也存在于精子鞭毛、粗纤维表面和纤维鞘上。这些发现表明，在公羊生殖细胞成熟过程中，膜联蛋白I和II从精子细胞顶体向精子结构和功能明确的部分输出。在公羊生殖细胞中未发现膜联蛋白III、IV和V。从睾丸和精子中分离出了膜联蛋白VI。在精子细胞中，发现它与内质网和线粒体相关，但顶体中没有。在精子中，它局限于鞭毛、线粒体、粗纤维表面和纤维鞘上。除钙调蛋白外，三种膜联蛋白在功能区域的存在可能表明精子控制和调节已知依赖钙的事件（如鞭毛运动、顶体反应和受精）的不同方式。
