Chajra H, Rousseau C F, Cortial D, Ronzière M C, Herbage D, Mallein-Gerin F, Freyria A M
IBCP, Institut de Biologie et Chimie des Protéines, Lyon, France.
Biomed Mater Eng. 2008;18(1 Suppl):S33-45.
Articular cartilage has a limited capacity for self-repair after trauma. Besides the conventional surgical techniques for repairing such defects, treatments involve implantation of autologous cells in suspension or within a variety of cell carrying scaffolds such as hyaluronic acid, alginate, agarose/alginate, fibrin or collagen. For the repair of full-thickness osteochondral defects, tissue engineers started to design single- or bi-phased scaffold constructs often containing hydroxyapatite-collagen composites, usually used as a bone substitute. The purpose of this study was to compare the behavior of bovine chondrocytes cultured in collagen-based scaffolds containing or not hydroxyapatite and cross-linked following two different methods. Calf chondrocytes seeded within Hemotèse and Collapat II sponges (SYMATESE biomaterials), chemically cross-linked with glutaraldehyde or EDC/NHS, were maintained up to one month in culture. The cells exhibited a similar behavior in the four scaffolds regarding proliferation level, deposition of glycosaminoglycans in the scaffolds and gene expression of types I, II and X collagens, aggrecan, MMP-1, -13 and the integrin subunits alpha10 and alpha11.
关节软骨在创伤后的自我修复能力有限。除了修复此类缺损的传统外科技术外，治疗方法还包括将自体细胞悬浮植入或植入各种细胞载体支架中，如透明质酸、藻酸盐、琼脂糖/藻酸盐、纤维蛋白或胶原蛋白。对于全层骨软骨缺损的修复，组织工程学家开始设计单相或双相支架结构，通常含有羟基磷灰石 - 胶原蛋白复合材料，常用作骨替代物。本研究的目的是比较在含有或不含羟基磷灰石且采用两种不同方法交联的胶原基支架中培养的牛软骨细胞的行为。接种在Hemotèse和Collapat II海绵（SYMATESE生物材料）内的小牛软骨细胞，用戊二醛或EDC/NHS进行化学交联，在培养中维持长达一个月。在四种支架中，细胞在增殖水平、支架中糖胺聚糖的沉积以及I型、II型和X型胶原蛋白、聚集蛋白聚糖、MMP - 1、 - 13以及整合素亚基α10和α11的基因表达方面表现出相似的行为。
