Sun Jun, Hou Xiao-Kui, Zheng Yu-Xin
Department of Orthopedics, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200021, China.
Department of Orthopedics, No. 9 Hospital Affiliated to Shanghai Jiaotong University, China.
Acta Orthop Traumatol Turc. 2016 Aug;50(4):464-9. doi: 10.1016/j.aott.2016.05.003. Epub 2016 Jul 16.
The aim of this study was to evaluate the efficacy of gene enhanced tissue engineering followed mosaicplasty in a goat model.
An acute cylindrical defect 9 mm in diameter was created in the weight bearing area of the medial femoral condyle in a goat model. Thirty-six medial femoral condyles were divided into 6 groups using different proportion of gene enhanced tissue engineering and mosaicplasty to restore the defects. The specimen received gross and histology observation, which was evaluated by the histological grading scale of O'Driscoll, Keeley and Salter. Transmission electron microscope observation was also performed. Two factors analysis of variance and Student-Newman-Kewls test were used to compare the specimen.
The gross and histology observation revealed that each defects of six groups had different restoration. The scores of the reparative tissue of three groups with gene enhancement were significantly higher than those in other three groups without gene enhancement (p > 0.05).
Gene enhanced tissue engineering followed mosaicplasty could restore a 9 mm diameter osteochondral defects in a goat model effectively. With the reduction of covering area of the graft, the advantages of the combined gene enhanced tissue engineering method can be better reflected.
本研究旨在评估基因强化组织工程联合镶嵌植骨术在山羊模型中的疗效。
在山羊模型的股骨内侧髁负重区制造一个直径9毫米的急性圆柱形缺损。将36个股骨内侧髁分成6组，采用不同比例的基因强化组织工程和镶嵌植骨术来修复缺损。对标本进行大体和组织学观察，采用O'Driscoll、Keeley和Salter的组织学分级标准进行评估。还进行了透射电子显微镜观察。使用双因素方差分析和Student-Newman-Kewls检验对标本进行比较。
大体和组织学观察显示，6组的每个缺损都有不同程度的修复。三组采用基因强化的修复组织评分显著高于其他三组未采用基因强化的组（p>0.05）。
基因强化组织工程联合镶嵌植骨术可有效修复山羊模型中直径9毫米的骨软骨缺损。随着移植物覆盖面积的减少，基因强化组织工程联合方法的优势能得到更好体现。
