Green-Church Kari B, Nichols Jason J
Campus Chemical Instrument Center Mass Spectrometry and Proteomics Facility, The Ohio State University, Columbus, OH 43210, USA.
Mol Vis. 2008 Feb 8;14:291-7.
The purpose of this report is to describe the contact lens deposition proteome associated with two silicone hydrogel contact lenses and care solutions using a mass spectrometric-based approach.
This was a randomized, controlled, examiner-masked crossover clinical trial that included 48 participants. Lenses and no-rub care solutions evaluated included galyfilcon A (Acuvue Advance, Vistakon Inc., Jacksonville, FL), lotrafilcon B (O2 Optix, CIBA Vision Inc., Duluth, GA), AQuify (CIBA Vision Inc.), and ReNu MoistureLoc (Bausch and Lomb Inc., Rochester, NY). After two weeks of daily wear in each lens-solution combination, the left lens was removed by the examiner (using gloves and forceps) and placed in a protein precipitation buffer (acetone). The precipitate was quantitated for total protein concentration (per lens), and proteins were then identified using liquid chromatography tandem mass spectrometry (nano-LC-MS/MS) and peptide sequencing.
Between 7.32 and 9.76 microg/lens of protein was observed on average from each lens-solution combination. There were 19 total unique proteins identified across the two lens materials, and six proteins were identified in all four lens-solution combinations including lipocalin, lysozyme, lacritin, lactoferrin, proline rich 4, and Ig Alpha. Lotrafilcon B was associated with 15 individual proteins (across both care solutions), and 53% of these proteins were observed in at least 50% of the analyses. Galyfilcon A was associated with 13 individual proteins, and 38.5% of these proteins were observed in at least 50% of the analyses. There were three unique proteins identified from galyfilcon A and four unique proteins identified from lotrafilcon B.
The total amount of proteins identified from silicone hydrogel materials is much less than the amount from traditional soft lens materials. For the most part, the deposition proteome across these lenses is similar, although the different polymer characteristics might be associated with some variability in observance of the less frequently identified proteins.
本报告旨在采用基于质谱的方法描述与两种硅水凝胶隐形眼镜及护理液相关的隐形眼镜沉积蛋白质组。
这是一项随机、对照、检查者盲法交叉临床试验，纳入了48名参与者。评估的镜片和免揉搓护理液包括加泪康A（爱尔康傲滴，威视康公司，佛罗里达州杰克逊维尔）、lotrafilcon B（O2 Optix，CIBA Vision公司，佐治亚州杜勒斯）、AQuify（CIBA Vision公司）和润明水凝护理液（博士伦公司，纽约州罗切斯特）。在每种镜片 - 护理液组合中每日佩戴两周后，检查者（使用手套和镊子）取下左眼镜片并置于蛋白质沉淀缓冲液（丙酮）中。对沉淀物进行总蛋白浓度定量（每片镜片），然后使用液相色谱串联质谱（纳升液相色谱 - 质谱/质谱）和肽测序鉴定蛋白质。
每种镜片 - 护理液组合平均每片镜片观察到7.32至9.76微克蛋白质。在两种镜片材料中总共鉴定出19种独特蛋白质，在所有四种镜片 - 护理液组合中鉴定出六种蛋白质，包括视黄醇结合蛋白、溶菌酶、泪腺素、乳铁蛋白、富含脯氨酸蛋白4和Igα。Lotrafilcon B与15种个体蛋白质相关（在两种护理液中），其中53%的蛋白质在至少50%的分析中被观察到。加泪康A与13种个体蛋白质相关，其中38.5%的蛋白质在至少50%的分析中被观察到。从加泪康A中鉴定出三种独特蛋白质，从lotrafilcon B中鉴定出四种独特蛋白质。
从硅水凝胶材料中鉴定出的蛋白质总量远少于传统软性镜片材料中的量。在很大程度上，这些镜片的沉积蛋白质组相似，尽管不同的聚合物特性可能与较少鉴定出的蛋白质的观察存在一些变异性有关。
