Zahorska-Markiewicz Barbara, Olszanecka-Glinianowicz Magdalena, Janowska Joanna, Kocełak Piotr
Department of Pathophysiology, Medical University of Silesia, Katowice, Poland.
Endokrynol Pol. 2008 Jan-Feb;59(1):18-22.
Apoptosis can influence both adipose tissue mass and its distribution. The suprafamily of tumour necrosis factor (TNF) receptors stimulate apoptosis. The aim of the study was to assess serum concentrations of tumour necrosis factor alpha (TNF-alpha), TNF soluble receptors (sTNFRs) and FAS in obese subjects and to examine the changes in these parameters after weight loss.
The study group consisted of 23 obese women without additional disease aged 36.6 +/- 10.9 years. These were examined before and after three-month weight reduction treatment consisting of a diet of 1000 kcal/day and physical exercise. The control group comprised 17 lean healthy women aged 40.3 +/- 5.5 years. Blood samples were taken in the morning after an overnight fast. Serum concentrations of TNF-alpha, sTNFRs and FAS were measured by enzyme linked immunosorbent assay (ELISA). Serum concentrations of insulin were measured by RIA. Serum concentrations of glucose, total cholesterol, HDL cholesterol and triglycerides were measured by an enzymatic procedure.
The mean weight loss over the three-month treatment was 11.4 +/- 3.1 kg. Following weight loss, serum TNF-alpha concentrations decreased significantly (7.3 +/- 3.0 vs. 5.4 +/- 1.6 pg/ml; p < 0.005) and concentrations of sTNFRs increased significantly (1222.6 +/- 211.8 vs. 1325.6 +/- 261.6 pg/ml; p < 0.05 and 1881.5 +/- 337.2 vs. 2057.4 +/- 358.7 pg/ml; p < 0.05 respectively). However, no changes in serum concentrations of FAS were observed after weight loss.
We observed increased serum concentrations of TNF-alpha but not of FAS in obese women. The concentrations of TNF decreased and those of sTNFRs increased after weight loss. However, the weight reduction therapy did not change serum concentrations of FAS.
细胞凋亡可影响脂肪组织的质量及其分布。肿瘤坏死因子（TNF）受体超家族可刺激细胞凋亡。本研究的目的是评估肥胖受试者血清中肿瘤坏死因子α（TNF-α）、TNF可溶性受体（sTNFRs）和FAS的浓度，并研究体重减轻后这些参数的变化。
研究组由23名无其他疾病的肥胖女性组成，年龄为36.6±10.9岁。她们在接受为期三个月的减肥治疗前后接受了检查，该治疗包括每天1000千卡的饮食和体育锻炼。对照组由17名年龄为40.3±5.5岁的瘦健康女性组成。在禁食过夜后的早晨采集血样。采用酶联免疫吸附测定（ELISA）法测定血清TNF-α、sTNFRs和FAS的浓度。采用放射免疫分析法（RIA）测定血清胰岛素浓度。采用酶法测定血清葡萄糖、总胆固醇、高密度脂蛋白胆固醇和甘油三酯的浓度。
三个月治疗期间的平均体重减轻为11.4±3.1千克。体重减轻后，血清TNF-α浓度显著降低（7.3±3.0对5.4±1.6皮克/毫升；p<0.005），sTNFRs浓度显著升高（1222.6±211.8对1325.6±261.6皮克/毫升；p<0.05以及1881.5±337.2对2057.4±358.7皮克/毫升；分别为p<0.05）。然而，体重减轻后未观察到血清FAS浓度的变化。
我们观察到肥胖女性血清中TNF-α浓度升高，但FAS浓度未升高。体重减轻后，TNF浓度降低，sTNFRs浓度升高。然而，减肥治疗并未改变血清FAS浓度。
