Tony H P, Lehrnbecher T, Merz H, Sebald W, Wilhelm M
Medizinische Poliklinik, University of Würzburg, F.R.G.
Leuk Res. 1991;15(10):911-9. doi: 10.1016/0145-2126(91)90167-r.
The responsiveness to IL-4 with and without costimulation with anti-IgM antibodies or phorbolester was studied in 35 cases of low grade non-Hodgkin lymphoma by analyzing enhancement of CD23 and HLA class II expression. The predominant phenotype responds directly to IL-4. Separate differentiation states can be distinguished according to coordinate or differential upregulation of CD23 and HLA class II molecules by IL-4 alone, and differences in responsiveness to anti-IgM antibodies. A particular subgroup of B-lymphoma cells defines a separate stage of B-cell differentiation. They fail to express high affinity binding sites for IL-4 and accordingly do not respond to IL-4-mediated signals. Cross-linking membrane IgM receptors or direct activation of protein kinase C via phorbolester induces IL-4 receptor expression and subsequent IL-4 reactivity.
通过分析CD23和HLA II类分子表达的增强情况，研究了35例低度非霍奇金淋巴瘤患者的细胞在有或无抗IgM抗体或佛波酯共刺激情况下对IL-4的反应性。主要表型直接对IL-4产生反应。根据单独的IL-4对CD23和HLA II类分子的协同或差异上调以及对抗IgM抗体反应性的差异，可以区分不同的分化状态。B淋巴瘤细胞的一个特定亚组定义了B细胞分化的一个单独阶段。它们不能表达IL-4的高亲和力结合位点，因此对IL-4介导的信号无反应。交联膜IgM受体或通过佛波酯直接激活蛋白激酶C可诱导IL-4受体表达及随后的IL-4反应性。
