Tony H P, Brückner A, Wilhelm M
Medizinische Poliklinik, University of Würzburg, Germany.
Hematol Pathol. 1993;7(2):79-90.
HLA-class II molecules can be induced in low-grade non-Hodgkin B lymphoma cells by either membrane IgM cross-linking or phorbolester stimulation. The ability of phorbolesters to substitute for anti-IgM antibodies in the activation of normal and malignant human B cells has been taken as evidence for the involvement of protein kinase C (PKC) in signals transduced through membrane IgM receptors (mIgR). Here we report on freshly isolated lymphoma B cells from different patients; the cells show a distinct regulation of HLA-class II expression. In certain lymphoma cases phorbol-myristate-acetate (PMA) not only fails to up-regulate HLA-class II molecules but also inhibits anti-IgM or interleukin-4 induced class II expression. This negative signal induced by PMA seems to operate specifically in HLA-class II regulation because PMA can induce other anti-IgM mediated events like blast transformation and induction of IL-4 responsiveness at the same time. Therefore these cells support the concept of functional heterogeneity in low-grade non-Hodgkin lymphoma and may represent a differentiation stage where anti-IgM antibodies and phorbolesters influence the regulation of HLA-class II expression in a contrary direction.
通过膜IgM交联或佛波酯刺激，可在低度非霍奇金B淋巴瘤细胞中诱导出HLA - II类分子。佛波酯在激活正常和恶性人B细胞时能够替代抗IgM抗体，这一能力被视为蛋白激酶C（PKC）参与通过膜IgM受体（mIgR）转导信号的证据。在此，我们报告从不同患者新鲜分离的淋巴瘤B细胞；这些细胞显示出HLA - II类表达的独特调控。在某些淋巴瘤病例中，佛波醇 - 肉豆蔻酸酯 - 乙酸酯（PMA）不仅未能上调HLA - II类分子，反而抑制抗IgM或白细胞介素 - 4诱导的II类表达。PMA诱导的这种负信号似乎在HLA - II类调控中具有特异性作用，因为PMA可同时诱导其他抗IgM介导的事件，如原始细胞转化和IL - 4反应性的诱导。因此，这些细胞支持低度非霍奇金淋巴瘤中功能异质性的概念，并且可能代表一个分化阶段，在这个阶段抗IgM抗体和佛波酯以相反方向影响HLA - II类表达的调控。
