Iaizzo P A, Wedel D J, Gallagher W J
Department of Anesthesiology, Mayo Clinic, Rochester, MN 55905.
Mayo Clin Proc. 1991 Oct;66(10):998-1004. doi: 10.1016/s0025-6196(12)61722-4.
The in vitro contracture test determines the sensitivity of freshly obtained skeletal muscle specimens to caffeine or halothane applied to a bathing solution. Muscles from persons susceptible to malignant hyperthermia have lower contracture thresholds for these agents than do normal muscle. Thus, known concentrations of these agents must be accurately administered. We describe a system in which the bath concentrations of halothane (0 to 3%) are reproducible within 0.2% halothane from month to month. With use of this system, which includes an on-line gas analyzer, the measured halothane concentration within the bathing solution was independent of the performance of the vaporizer. The system is inexpensive and stable. The methods used to determine the bath concentrations of halothane and caffeine are reviewed. Finally, experimental equipment and a new recording system are described.
体外挛缩试验可测定新鲜获取的骨骼肌标本对添加于浴液中的咖啡因或氟烷的敏感性。与正常肌肉相比，易患恶性高热者的肌肉对这些药物的挛缩阈值更低。因此，必须准确给予已知浓度的这些药物。我们描述了一种系统，在该系统中，氟烷的浴液浓度（0%至3%）每月的再现性在0.2%氟烷范围内。使用该系统（包括在线气体分析仪）时，浴液中测得的氟烷浓度与蒸发器的性能无关。该系统价格低廉且稳定。本文回顾了用于测定氟烷和咖啡因浴液浓度的方法。最后，介绍了实验设备和一种新的记录系统。
