Keskin Goksal, Inal Ali, Keskin Dilek, Pekel Aysel, Baysal Ozan, Dizer Ufuk, Sengül Ali
Department of Immunology, Dişkapi Training and Research Hospital, Ankara, Turkey.
Protein Pept Lett. 2008;15(3):314-7. doi: 10.2174/092986608783744153.
Several autoantibodies found in RA are directed to epitopes in citrullinated proteins. One of them is anti modified citrullinated vimentin (Anti-MCV). We tested the value a newly developed ELISA for the detection of antibodies against a genetically modified citrullinated vimentin (anti-MCV) in comparison with an anti-CCP based ELISA system for the diagnosis of RA. Thirty-five patients with RA (mean age; 42.6 +/- 10.87 years, mean disease duration; 9.37 +/- 3.98 years) were enrolled in this study. Twenty -five ankylosing spondylitis (mean age; 35.88 +/- 6.64 years, mean disease duration; 10.25 +/- 4.61 years), and 19 healthy subjects (mean age; 40.26 +/- 5.11 years) served as controls. Anti-CCP antibodies and Anti-MCV antibodies were measured using ELISA. In all RA patients, mean anti- CCP level was 69.07 +/- 90.43 U/ml and anti-MCV level was 665.77 +/- 1040.19 U/ml. In patients with AS, the mean anti-CCP level was 10.7 +/- 5.22 U/ml and anti-MCV level was 40.54 +/- 20.15 U/ml. In healthy controls, the mean anti-CCP level was 11.11 +/- 7.65 U/ml, anti-MCV level was 23.12 +/- 12.04 U/ml. In patients with active RA, the mean serum anti-CCP level was 100.54 +/- 98.07 U/ml and anti-MCV level was 998.74 +/- 1154.93 U/ml. In patients with inactive RA, the mean serum anti-CCP level was 8.77 +/- 1.55 U/ml and anti-MCV level was 27.59 +/- 23.10 U/ml. According to these results; In patients with RA, the mean serum anti-MCV and anti-CCP levels were significantly high compared to patients with AS and healthy controls (p=0.002, p=0.001, p=0.002, p=0.001 respectively). The mean serum anti-MCV and anti- CCP levels were significantly higher in active patients with RA than in inactive patients with RA patients (p=0.001 and p=0.001 respectively). In inactive patients with RA, the mean serum anti-MCV and anti-CCP levels were similar in patients with AS and patients (p=0.484, p=0.308, p=0.09 and p=0.222 respectively). The mean serum anti-MCV levels were correlated with DAS 28 (r=0.531, p=0.001), VAS score (r=0.332, p=0.01), ESR (r=0.458, p=0.001), serum CRP levels (r=0.568, p=0.01), serum RF levels (r=0.529, p=0.001), swollen joints number (r=0.525, p=0.001) and tender joints number (r=0.638, p=0.001). As a result; measurement of serum anti-MCV levels is useful for diagnosis of RA and combined use of anti-MCV and RF may be more useful prognostic factor than either method alone, RF and anti-CCP.
类风湿关节炎（RA）中发现的几种自身抗体针对瓜氨酸化蛋白的表位。其中之一是抗修饰瓜氨酸波形蛋白（Anti-MCV）。我们测试了一种新开发的酶联免疫吸附测定（ELISA）法检测针对基因工程瓜氨酸化波形蛋白的抗体（抗MCV）的价值，并与基于抗环瓜氨酸肽（anti-CCP）的ELISA系统用于RA的诊断进行比较。本研究纳入了35例RA患者（平均年龄42.6±10.87岁，平均病程9.37±3.98年）。25例强直性脊柱炎患者（平均年龄35.88±6.64岁，平均病程10.25±4.61年）和19名健康受试者（平均年龄40.26±5.11岁）作为对照。采用ELISA法检测抗CCP抗体和抗MCV抗体。在所有RA患者中，抗CCP平均水平为69.07±90.43 U/ml，抗MCV水平为665.77±1040.19 U/ml。在AS患者中，抗CCP平均水平为10.7±5.22 U/ml，抗MCV水平为40.54±20.15 U/ml。在健康对照中，抗CCP平均水平为11.11±7.65 U/ml，抗MCV水平为23.12±12.04 U/ml。在活动期RA患者中，血清抗CCP平均水平为100.54±98.07 U/ml，抗MCV水平为998.74±1154.93 U/ml。在非活动期RA患者中，血清抗CCP平均水平为8.77±1.55 U/ml，抗MCV水平为27.59±23.10 U/ml。根据这些结果；与AS患者和健康对照相比，RA患者血清抗MCV和抗CCP平均水平显著升高（分别为p=0.002、p=0.001、p=0.002、p=0.001）。活动期RA患者血清抗MCV和抗CCP平均水平显著高于非活动期RA患者（分别为p=0.001和p=0.001）。在非活动期RA患者中，AS患者与RA患者血清抗MCV和抗CCP平均水平相似（分别为p=0.484、p=0.308、p=0.09和p=0.222）。血清抗MCV平均水平与疾病活动度评分28（DAS 28）相关（r=0.531，p=0.001）、视觉模拟评分（VAS评分）（r=0.332，p=0.01）、红细胞沉降率（ESR）（r=0.458，p=0.001）、血清C反应蛋白（CRP）水平（r=0.568，p=0.01）、血清类风湿因子（RF）水平（r=0.529，p=0.001）、肿胀关节数（r=0.525，p=0.001）和压痛关节数（r=0.638，p=0.001）。结果；血清抗MCV水平的检测对RA的诊断有用，抗MCV与RF联合使用可能比单独使用RF及抗CCP更有助于判断预后。
