Department of Rheumatology and Internal Medicine, Wroclaw University Hospital, Wroclaw, Poland.
Department of Rheumatology and Internal Medicine, Wroclaw Medical University, Wroclaw, Poland.
Clin Rheumatol. 2019 Mar;38(3):827-834. doi: 10.1007/s10067-018-4326-5. Epub 2018 Nov 7.
The aim of the study was to evaluate the frequency of anti-mutated citrullinated vimentin antibodies (a-Sa), anti-citrullinated α-enolase peptide 1 antibodies (a-CEP-1), anti-filaggrin antibodies (AFAs), heterogeneous nuclear ribonucleoprotein compies/anti-RA33-antibodies (a-hnRNP/RA33), anti-carbamylated protein antibodies (a-CarP), and metalloproteinase (MMPs) activity in patients with early inflammatory arthritis (EIA).
Seventy-four patients with EIA: 51 diagnosed with RA (rheumatoid arthritis) and 23 with UA (undifferentiated arthritis), and 20 healthy volunteers were enrolled to the study. Inflammatory markers, rheumatoid factor (RF), and antibodies mentioned above were assessed in all patients.
In the EIA group, we observed significantly higher concentration of a-CEP-1 (65.8 ± 111.6 RU/mL) than in controls (2.0 ± 0.0 RU/mL). In RF(+) RA patients, we observed higher concentration of a-Sa and a-CEP-1 than in other groups. A-Sa were positive in 69% of RF(+) RA, 37% of RF(-) RA, 26% of UA patients and in 10% of controls. A-CEP-1 were positive in 77% of RF(+) RA patients, in 56% of RF(-) RA patients, in 8.7% of UA patients, but they were negative in controls. In patients with RF(+) RA, positive a-CarP were present statistically significantly more often than in RF (-) RA patients. No statistically significant difference in frequency of a-hnRNP/RA33 and AFA between RF(+) RA, RF(-) RA, and UA was observed.
Our results suggest that a-CEP-1 may help in differentiation between RF(-) RA and UA. a-CEP-1 and a-Sa may be useful while diagnosing EIA. a-CarP may be used in differentiation of RA RF(-) and UA. However, a follow-up study is needed to evaluate the prognostic value of analyzed antibodies.
本研究旨在评估抗突变型瓜氨酸波形蛋白抗体（a-Sa）、抗瓜氨酸α-烯醇化酶肽 1 抗体（a-CEP-1）、抗丝聚蛋白抗体（AFAs）、异质核核糖核蛋白复合物/抗 RA33 抗体（a-hnRNP/RA33）、抗氨甲酰化蛋白抗体（a-CarP）和金属蛋白酶（MMPs）在早期炎症性关节炎（EIA）患者中的频率。
本研究纳入了 74 例 EIA 患者：51 例确诊为类风湿关节炎（RA），23 例为未分化关节炎（UA），20 例健康志愿者。所有患者均检测了炎症标志物、类风湿因子（RF）和上述抗体。
在 EIA 组中，我们观察到 a-CEP-1 的浓度明显高于对照组（2.0±0.0 RU/mL）（65.8±111.6 RU/mL）。在 RF(+) RA 患者中，我们观察到 a-Sa 和 a-CEP-1 的浓度高于其他组。a-Sa 在 69%的 RF(+) RA、37%的 RF(-) RA、26%的 UA 患者和 10%的对照组中呈阳性。a-CEP-1 在 77%的 RF(+) RA 患者、56%的 RF(-) RA 患者、8.7%的 UA 患者中呈阳性，但在对照组中为阴性。在 RF(+) RA 患者中，阳性 a-CarP 的出现频率明显高于 RF(-) RA 患者。RF(+) RA、RF(-) RA 和 UA 之间 a-hnRNP/RA33 和 AFA 的频率无统计学差异。
我们的研究结果表明，a-CEP-1 可能有助于区分 RF(-) RA 和 UA。a-CEP-1 和 a-Sa 可能有助于 EIA 的诊断。a-CarP 可用于区分 RA RF(-) 和 UA。然而，需要进一步的研究来评估分析抗体的预后价值。
