血清结核特异性抗原抗体在结核病诊断中的临床意义

[The clinical significance of serum tuberculosis specific antigen antibody in the diagnosis of tuberculosis].

作者信息

Gao Meng-Qiu, Chu Nai-Hui, Wang Hai-Ying, Zhao Qing-Rong, Li Hua, Gao Xu-Sheng, Luo Tao

机构信息

Department of Tuberculosis, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2007 Dec;30(12):918-20.

PMID:18336768

Abstract

OBJECTIVE

To evaluate the significance of serum tuberculosis specific antigen (TB-SA) antibody detection in the diagnosis of tuberculosis.

METHODS

TB-SA antibody in the serum samples from 829 cases of tuberculosis, 278 patients with non-tuberculosis lung diseases and 125 healthy volunteers were detected using enzyme-linked immunosorbent assay (ELISA). Tuberculosis was confirmed by clinical, bacteriology, X-ray examination and pathology studies.

RESULTS

The sensitivity of TB-SA antibody in diagnosis of bacteriologically positive and negative pulmonary tuberculosis and extrapulmonary tuberculosis was 75.1% (272/362), 68.9% (226/328) and 71.2% (99/139), respectively; the combined sensitivity was 72.0% (597/829), and the specificity 82.1% (331/403). The relationship between the OD(405) of TB-SA antibody and PPD positivity showed no linear relationship, suggesting that BCG vaccination did not affect the value of serum TB-SA antibody.

CONCLUSION

The results suggest that measurement of serum TB-SA is a relatively sensitive and specific method for the diagnosis and differential diagnosis of tuberculosis.

摘要

目的

评估血清结核特异性抗原（TB-SA）抗体检测在结核病诊断中的意义。

方法

采用酶联免疫吸附试验（ELISA）检测829例结核病患者、278例非结核性肺部疾病患者及125名健康志愿者血清样本中的TB-SA抗体。结核病经临床、细菌学、X线检查及病理学研究确诊。

结果

TB-SA抗体诊断菌阳和菌阴肺结核及肺外结核的敏感性分别为75.1%（272/362）、68.9%（226/328）和71.2%（99/139）；联合敏感性为72.0%（597/829），特异性为82.1%（331/403）。TB-SA抗体OD(405)与PPD阳性之间无线性关系，提示卡介苗接种不影响血清TB-SA抗体值。