鹅绒委陵菜三萜皂苷化合物的抗乙型肝炎病毒活性
Anti-hepatitis B virus activities of triterpenoid saponin compound from Potentilla anserine L.
作者信息
Zhao Y-L, Cai G-M, Hong X, Shan L-M, Xiao X-H
机构信息
Institute of Traditional Chinese Material Medica, People's Liberation Army, Beijing 100039, PR China.
出版信息
Phytomedicine. 2008 Apr;15(4):253-8. doi: 10.1016/j.phymed.2008.01.005. Epub 2008 Mar 11.
The Tibetan herb Potentilla anserina L. has been widely used in China for many thousands of years to treat hepatitis-B. Bioassay-guided fractionation of the ethanol extract of the rhizomes led to the isolation of a triterpenoid saponin (TS) that was determined to be 2alpha,3beta,19alpha-trihydroxyurs-12-en-28-oic acid beta-D-glucopyranosyl ester. Using models of HBV infection, this compound was evaluated for its effect on HBV antigene expression in the 2.2.15 cell line in vitro and anti-hepatitis B virus (HBV) activities in Peking ducklings in vivo. Results showed that it could decrease the expression levels of HBsAg, HBeAg and HBVDNA in the 2.2.15 cell culture and the inhibitory effect was not due to the cytotoxity of the triterpenoid saponin. The antiviral study in vivo on Peking ducklings also demonstrated that this compound inhibits duck hepatitis B virus (DHBV) DNA replication.
藏药蕨麻在中国已被广泛使用了数千年，用于治疗乙型肝炎。对根茎乙醇提取物进行生物活性导向分离，得到一种三萜皂苷（TS），经鉴定为2α，3β，19α-三羟基乌苏-12-烯-28-酸β-D-吡喃葡萄糖酯。利用乙肝病毒感染模型，在体外2.2.15细胞系中评估该化合物对乙肝病毒抗原表达的影响，并在体内北京鸭中评估其抗乙肝病毒（HBV）活性。结果表明，它可降低2.2.15细胞培养物中HBsAg、HBeAg和HBVDNA的表达水平，且这种抑制作用并非由于三萜皂苷的细胞毒性。对北京鸭的体内抗病毒研究也表明，该化合物可抑制鸭乙肝病毒（DHBV）DNA复制。
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