García Miriam Martin, De Rissio Ana Maria, Villalonga Ximena, Mengoni Eleonora, Cardoni Rita L
Instituto Nacional de Parasitología Dr. M. Fatala Chabén, Administración Nacional de Laboratorios e Institutos de Salud Dr. CG Malbrán, Buenos Aires, Argentina.
Am J Trop Med Hyg. 2008 Mar;78(3):499-503.
Most congenital transmissions of Trypanosoma cruzi are not detected. As the levels of mediators regulating the immune response might be different in the absence or in the presence of transmission, we explored the levels of tumor necrosis factor (TNF) and soluble TNF receptors TNF-R1 and -R2 in T. cruzi-infected pregnant women and the neonates. We previously found that the circulating levels of TNF were higher in non-transmitting than in transmitting pregnant women. This observation has now been extended to the spontaneous release of TNF by peripheral blood leukocytes (PBLs) that was also higher in non-transmitting than in transmitting pregnant women. As their mothers, non-infected neonates had higher circulating levels of TNF than congenitally infected children. The circulating levels of sTNF-R1 increased in non-transmitting and transmitting mothers and in infected and non-infected neonates. The circulating levels of sTNF-R2 were approximately 60% higher in infected than in non-infected neonates (1,635 +/- 101 and 1,027 +/- 100 pg/mL, respectively) and remained higher at 1 year of age. This important increase, only observed in infected neonates, could be useful to orientate to the presence of vertical transmission of T. cruzi infection.
大多数克氏锥虫的先天性传播未被检测到。由于在传播存在或不存在的情况下调节免疫反应的介质水平可能不同，我们研究了克氏锥虫感染的孕妇和新生儿中肿瘤坏死因子（TNF）以及可溶性TNF受体TNF-R1和TNF-R2的水平。我们之前发现，未发生传播的孕妇中TNF的循环水平高于发生传播的孕妇。现在这一观察结果已扩展到外周血白细胞（PBL）自发释放的TNF，未发生传播的孕妇中该水平也高于发生传播的孕妇。与她们的母亲一样，未感染的新生儿的TNF循环水平高于先天性感染的儿童。未发生传播和发生传播的母亲以及感染和未感染的新生儿中，sTNF-R1的循环水平均升高。感染新生儿的sTNF-R2循环水平比未感染新生儿高约60%（分别为1635±101和1027±100 pg/mL），且在1岁时仍较高。这种仅在感染新生儿中观察到的显著升高，可能有助于确定克氏锥虫感染垂直传播的存在。
