Rho Jungmin, Ryu Jong Soon, Hur Wonhee, Kim Chang Wook, Jang Jeong Won, Bae Si Hyun, Choi Jong Young, Jang Sung Key, Yoon Seung Kew
Panbionet, Corp., POSTECH Biotech Center, Pohang 790-784, Republic of Korea.
J Microbiol. 2008 Feb;46(1):81-7. doi: 10.1007/s12275-007-0121-8.
The genotype of the hepatitis C virus (HCV) strain infecting a given patient is an important predictive factor for the clinical outcome of chronic liver disease and its response to anti-viral therapeutic agents. We herein sought to develop a new easy, sensitive and accurate HCV genotyping method using annealing genotype-specific capture probes (AGSCP) in an automation-friendly 96-well plate format. The validation of our new AGSCP was performed using the Standard HCV Genotype Panel. We then used both our AGSCP and the commercially available INNO-LiPA assay to analyze the HCV genotypes from 111 Korean patients. Discordant results were analyzed by direct sequencing. AGSCP successfully genotyped the standard panel. The genotypes of 111 patient samples were also obtained successfully by AGSCP and INNO-LiPA. We observed a high concordance rate (93 matched samples, 83.8%) between the two assays. Sequencing analysis of the 18 discordant results revealed that the AGSCP had correctly identified 12 samples, whereas the INNO-LiPA had correctly identified only 6. These results collectively indicate that AGSCP assay is a convenient and sensitive method for large-scale genotyping, and it may be a promising tool for the determination of HCV and other genotypes in clinical settings.
感染特定患者的丙型肝炎病毒（HCV）毒株的基因型，是慢性肝病临床结局及其对抗病毒治疗药物反应的重要预测因素。我们在此寻求开发一种新的简便、灵敏且准确的HCV基因分型方法，该方法使用基因型特异性捕获探针（AGSCP），采用便于自动化操作的96孔板形式。我们使用标准HCV基因型检测板对新的AGSCP进行了验证。然后，我们使用AGSCP和市售的INNO-LiPA检测法对111例韩国患者的HCV基因型进行分析。对不一致的结果进行直接测序分析。AGSCP成功地对标准检测板进行了基因分型。通过AGSCP和INNO-LiPA也成功获得了111例患者样本的基因型。我们观察到两种检测方法之间的一致性率很高（93个匹配样本，83.8%）。对18个不一致结果的测序分析表明，AGSCP正确鉴定了12个样本，而INNO-LiPA仅正确鉴定了6个。这些结果共同表明，AGSCP检测法是一种用于大规模基因分型的便捷且灵敏的方法，它可能是临床环境中确定HCV及其他基因型的一种有前景的工具。
