Kelly William J
Department of Chemical Engineering, Villanova University, 800 Lancaster Avenue, Villanova, PA 19085, USA.
Biotechnol Appl Biochem. 2008 Apr;49(Pt 4):225-38. doi: 10.1042/BA20070177.
CFD (computational fluid dynamics) has been used to model upstream bioprocessing steps such as fermentation and homogenization. The focus of these studies has oftentimes been to characterize single-phase (liquid) flow and hydrodynamic shear. In the actual bioprocessing operations, however, there are at least two phases (cells and liquid) present. In the bioreactor, the gas bubbles constitute a third phase. More recent CFD models have considered the momentum and mass transfer that occurs between the phases. This review summarizes studies from the biochemical and biomedical literature relating to the use of CFD to model the performance of a variety of bioreactor types. Particular emphasis will be placed on describing current methods for handling multi-phase flow involving animal cells and/or gas bubbles.
计算流体动力学（CFD）已被用于对发酵和均质化等上游生物处理步骤进行建模。这些研究的重点通常是表征单相（液体）流动和流体动力剪切。然而，在实际的生物处理操作中，至少存在两相（细胞和液体）。在生物反应器中，气泡构成第三相。最近的CFD模型已经考虑了相间发生的动量和质量传递。本综述总结了生化和生物医学文献中有关使用CFD对各种类型生物反应器性能进行建模的研究。将特别强调描述处理涉及动物细胞和/或气泡的多相流的当前方法。
