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SrfB是转录因子血清反应因子家族的成员之一，可调节盘基网柄菌中的饥饿反应和早期发育。

SrfB, a member of the Serum Response Factor family of transcription factors, regulates starvation response and early development in Dictyostelium.

作者信息

Galardi-Castilla María, Pergolizzi Barbara, Bloomfield Gareth, Skelton Jason, Ivens Al, Kay Robert R, Bozzaro Salvatore, Sastre Leandro

机构信息

Instituto de Investigaciones Biomédicas CSIC/UAM. Arturo Duperier, 4. 28029 Madrid, Spain.

出版信息

Dev Biol. 2008 Apr 15;316(2):260-74. doi: 10.1016/j.ydbio.2008.01.026. Epub 2008 Jan 31.

DOI:10.1016/j.ydbio.2008.01.026
PMID:18339368
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3819988/
Abstract

The Serum Response Factor (SRF) is an important regulator of cell proliferation and differentiation. Dictyostelium discoideum srfB gene codes for an SRF homologue and is expressed in vegetative cells and during development under the control of three alternative promoters, which show different cell-type specific patterns of expression. The two more proximal promoters directed gene transcription in prestalk AB, stalk and lower-cup cells. The generation of a strain where the srfB gene has been interrupted (srfB(-)) has shown that this gene is required for regulation of actin-cytoskeleton-related functions, such as cytokinesis and macropinocytosis. The mutant failed to develop well in suspension, but could be rescued by cAMP pulsing, suggesting a defect in cAMP signaling. srfB(-) cells showed impaired chemotaxis to cAMP and defective lateral pseudopodium inhibition. Nevertheless, srfB(-) cells aggregated on agar plates and nitrocellulose filters 2 h earlier than wild type cells, and completed development, showing an increased tendency to form slug structures. Analysis of wild type and srfB(-) strains detected significant differences in the regulation of gene expression upon starvation. Genes coding for lysosomal and ribosomal proteins, developmentally-regulated genes, and some genes coding for proteins involved in cytoskeleton regulation were deregulated during the first stages of development.

摘要

血清反应因子（SRF）是细胞增殖和分化的重要调节因子。盘基网柄菌的srfB基因编码一种SRF同源物，在营养细胞中以及发育过程中，在三个可变启动子的控制下表达，这三个启动子表现出不同的细胞类型特异性表达模式。两个更近端的启动子指导前柄AB细胞、柄细胞和下杯细胞中的基因转录。一个srfB基因被中断的菌株（srfB(-)）的产生表明，该基因是调节肌动蛋白细胞骨架相关功能（如胞质分裂和巨胞饮作用）所必需的。该突变体在悬浮培养中发育不良，但可通过cAMP脉冲处理得到挽救，这表明其cAMP信号传导存在缺陷。srfB(-)细胞对cAMP的趋化性受损，侧向伪足抑制存在缺陷。然而，srfB(-)细胞在琼脂平板和硝酸纤维素滤膜上比野生型细胞提前2小时聚集，并完成发育，形成蛞蝓状结构的趋势增加。对野生型和srfB(-)菌株的分析发现，饥饿时基因表达调控存在显著差异。编码溶酶体和核糖体蛋白的基因、发育调控基因以及一些编码参与细胞骨架调控的蛋白质的基因在发育的第一阶段表达失调。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/010c8ebbd81a/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/1e67509896d4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/7b67180d15ab/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/a9d34396cbbf/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/10e2f6f7a586/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/08f867075877/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/12b345347e72/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/037dc4b7a41f/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/fd1393dca952/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/9dcb4e5268e3/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/010c8ebbd81a/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/1e67509896d4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/7b67180d15ab/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/a9d34396cbbf/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/10e2f6f7a586/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/08f867075877/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/12b345347e72/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/037dc4b7a41f/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/fd1393dca952/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/9dcb4e5268e3/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a30a/3819988/010c8ebbd81a/gr10.jpg

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