Roussan D A, Totanji W S, Khawaldeh G Y
Provimi Jordan, P.O. Box 499, Amman 11118, Jordan.
Poult Sci. 2008 Apr;87(4):661-4. doi: 10.3382/ps.2007-00509.
Infectious bronchitis virus (IBV) causes respiratory disease in chickens all over the world. Infectious bronchitis virus has many serotypes that do not confer cross protection against each other. The current study was designed to know which IBV types were circulating in Jordanian broiler chickens. Tracheal swabs from 175 broiler flocks at the acute phase of respiratory disease were collected. The swabs were subjected to RNA extraction and tested by reverse transcription PCR (RT-PCR). Specific-nested PCR were performed on RT-PCR products to detect and differentiate strains of Massachusetts, 4/91, and D274 types. The nucleic acid of IBV was detected in 105 out 174 (60%) broiler flocks by RT-PCR. Specific-nested PCR revealed that 35.2, 31.4, and 8.6% of these flocks had Massachusetts, 4/91, and D274, respectively, alone. In 24.8% of tested flocks, 2 types of IBV were detected. However, because the primers used in this study were designed specifically for 3 types of IBV, other types might have been present but not detected. Future work should include the isolation and molecular characterization of IBV in the region to adopt a suitable vaccination program using the common field serotypes as vaccines to protect against IBV-caused disease.
传染性支气管炎病毒（IBV）在全球范围内引发鸡的呼吸道疾病。传染性支气管炎病毒有许多血清型，它们之间不存在交叉保护作用。本研究旨在了解约旦肉鸡中传播的是哪些IBV类型。收集了175个处于呼吸道疾病急性期的肉鸡群的气管拭子。对拭子进行RNA提取，并通过逆转录聚合酶链反应（RT-PCR）进行检测。对RT-PCR产物进行特异性巢式PCR，以检测和区分马萨诸塞型、4/91型和D274型毒株。通过RT-PCR在174个肉鸡群中的105个（60%）中检测到了IBV核酸。特异性巢式PCR显示，这些鸡群中分别有35.2%、31.4%和8.6%单独感染了马萨诸塞型、4/91型和D274型。在24.8%的检测鸡群中，检测到了2种IBV类型。然而，由于本研究中使用的引物是专门针对3种IBV类型设计的，可能存在其他未被检测到的类型。未来的工作应包括对该地区IBV进行分离和分子特征分析，以采用合适的疫苗接种方案，使用常见的田间血清型作为疫苗来预防IBV引起的疾病。
