Gutierrez del Alamo A, Verstegen M W A, Den Hartog L A, Perez de Ayala P, Villamide M J
Nutreco Poultry and Rabbit Research Centre, 45950 Casarrubios del Monte, Toledo, Spain.
Poult Sci. 2008 Apr;87(4):759-67. doi: 10.3382/ps.2007-00437.
A total of 5,000 one-day-old male broiler chickens were assigned to 8 different treatments in a 4 x 2 factorial design. Four wheat cultivars (Amiro, Guadalupe, Isengrain, and Horzal) and 2 levels (0 or 1 kg/t of feed) of an enzyme cocktail (Avizyme 1300, xylanase, 2,500 U/kg and protease, 800 U/kg) were used. Nutritionally complete mash diets contained 65 and 70% of the test wheat for the starter and grower period, respectively. Test wheats were used in diets for broilers, and growth performance and AME contents were measured. Broiler performance was measured in 4,800 broilers allocated to floor pens with 75 birds each and fed from 1 to 42 d of age. Digestibilities and AME contents of diets were measured in 200 broilers from 6 to 27 d of age individually allocated to battery cages. Chromic oxide (Cr(2)O(3)) at an inclusion rate of 0.5% in the diet was used as an indigestible marker. Apparent metabolizable energy was corrected by zero N balance to obtain AME(n). Wheat cultivar strongly influenced animal performance during the starter period (1 to 21 d of age). During the grower period (21 to 42 d of age), only BW and daily feed intake were influenced by wheat cultivar. Differences in daily feed intake were associated with differences in AME(n) intake during the starter period, but not during the grower period. Nutrient digestibility was higher with the use of enzyme. Animal performance was not affected (i.e., wheat cultivar differences were not eliminated by using enzymes). During the grower period, significant interactions were detected with regard to nutrient digestibility and AME(n). Differences in AME(n) content of wheat could not be explained by digestible starch.
总共5000只1日龄雄性肉鸡被分配到4×2析因设计的8种不同处理中。使用了4个小麦品种(阿米罗、瓜达卢佩、伊森格雷恩和霍尔扎尔)和2个水平(0或1千克/吨饲料)的酶混合物(阿维酶1300,木聚糖酶,2500单位/千克和蛋白酶,800单位/千克)。营养完全的粉料日粮在育雏期和生长育肥期分别含有65%和70%的试验小麦。试验小麦用于肉鸡日粮,并测定生长性能和表观代谢能(AME)含量。在分配到地面围栏、每栏75只鸡并从1日龄饲养至42日龄的4800只肉鸡中测定生长性能。在单独分配到层叠笼中、6日龄至27日龄的200只肉鸡中测定日粮的消化率和AME含量。日粮中添加0.5%的氧化铬(Cr₂O₃)作为不可消化标记物。表观代谢能通过零氮平衡进行校正以获得氮校正表观代谢能(AME(n))。小麦品种在育雏期(1日龄至21日龄)对动物性能有强烈影响。在生长育肥期(21日龄至42日龄),只有体重和日采食量受小麦品种影响。日采食量的差异与育雏期AME(n)摄入量的差异有关,但与生长育肥期无关。使用酶后营养物质消化率更高。动物性能未受影响(即使用酶并未消除小麦品种差异)。在生长育肥期,在营养物质消化率和AME(n)方面检测到显著的交互作用。小麦AME(n)含量的差异无法用可消化淀粉来解释。