Bridge P D, Schlitt T, Cannon P F, Buddie A G, Baker M, Borman A M
Biological Sciences Division, British Antarctic Survey, High Cross, Madingley Road, Cambridge CB3 0ET, UK.
Mycopathologia. 2008 Jul;166(1):1-16. doi: 10.1007/s11046-008-9094-3. Epub 2008 Mar 14.
The hypothesis that ITS structural features can be used to define fungal groups, where sequence analysis is unsatisfactory, was examined in plant and animal pathogenic fungi. Structural models of ITS1 regions were predicted for presumed closely related species in Colletotrichum and Trichophyton anamorphs of Arthroderma species. Structural alignment of models and comparison with ITS sequence analysis identified a variable region in a conserved hairpin formed from a common inverted repeat. Thirteen different hairpin structure models were obtained for Colletotrichum species and five different models were obtained for Trichophyton species. The different structure types could be matched to individual species and species complexes as defined by ITS sequence analysis.
即在内转录间隔区（ITS）序列分析结果不理想的情况下，其结构特征可用于定义真菌类群。针对炭疽菌属以及节皮菌属物种的须癣毛癣菌变种中假定的近缘物种，预测了ITS1区域的结构模型。对模型进行结构比对并与ITS序列分析结果相比较，在由一个常见反向重复序列形成的保守发夹结构中确定了一个可变区。炭疽菌属物种获得了13种不同的发夹结构模型，须癣毛癣菌物种获得了5种不同的模型。不同的结构类型可与根据ITS序列分析定义的各个物种及物种复合体相匹配。
