Pandey K N, Orgebin-Crist M C
Department of Biochemistry and Molecular Biology, Medical College of Georgia School of Medicine, Augusta 30912.
Biochem Biophys Res Commun. 1991 Oct 15;180(1):437-44. doi: 10.1016/s0006-291x(05)81312-9.
Immunoreactive atrial natriuretic factor (ANF) was localized by immunochemistry and radioimmunoassay in mouse and rat testes. The analyses of acid extracts of testes by gel filtration and reverse phase high pressure liquid chromatography (HPLC) revealed the presence of a processed 31-residues peptide and the precursor form of 126-residues pro-ANF molecule corresponding to a molecular weights (Mr) of 3,300 and 18,000, respectively. The concentration of ANF in mice testis averaged 12 +/- 3 ng and in rat testis 8 +/- 2 ng per g of tissue. Specific immunochemical staining was localized in the spermatids and elongating spermatozoa of mammalian testis. The demonstration of immunoreactive ANF in testis and specific localization in spermatids reveals a new site at which ANF may be actively synthesized and regulate paracrine and/or autocrine function(s) during spermiogenesis, suggesting a broader spectrum of ANF action in addition to its known regulatory role in the control of blood pressure homeostasis.
通过免疫化学和放射免疫测定法对小鼠和大鼠睾丸中的免疫反应性心钠素（ANF）进行了定位。通过凝胶过滤和反相高压液相色谱（HPLC）对睾丸酸性提取物的分析显示，存在一种加工后的31个氨基酸残基的肽以及126个氨基酸残基的前心钠素分子的前体形式，其分子量（Mr）分别为3300和18000。小鼠睾丸中ANF的浓度平均为每克组织12±3纳克，大鼠睾丸中为8±2纳克。特异性免疫化学染色定位于哺乳动物睾丸的精子细胞和伸长的精子中。睾丸中免疫反应性ANF的证实及其在精子细胞中的特异性定位揭示了一个新的位点，在精子发生过程中ANF可能在此处被积极合成并调节旁分泌和/或自分泌功能，这表明除了其在控制血压稳态方面已知的调节作用外，ANF的作用谱更广泛。
