Vosegaard Thomas, Bertelsen Kresten, Pedersen Jan M, Thøgersen Lea, Schiøtt Birgit, Tajkhorshid Emad, Skrydstrup Troels, Nielsen Niels Chr
Center for Insoluble Protein Structures and Department of Chemistry, University of Aarhus, Langelandsgade 140, DK-8000 Aarhus C, Denmark.
J Am Chem Soc. 2008 Apr 16;130(15):5028-9. doi: 10.1021/ja8000612. Epub 2008 Mar 15.
We demonstrate that a significant improvement in the spectral resolution may be achieved in solid-state NMR experiments of proteins in inhomogeneously disordered oriented lipid bilayers. Using 1H homonuclear decoupling instead of standard 1H heteronuclear decoupling, the 15N line widths may be reduced by up to seven times for such samples. For large oriented membrane proteins, such resolution enhancements may be crucial for assignment and structural interpretation.
我们证明,在非均匀无序取向脂质双层中蛋白质的固态核磁共振实验中,可以实现光谱分辨率的显著提高。对于此类样品,使用1H同核去耦而非标准的1H异核去耦,15N线宽可减少多达七倍。对于大型取向膜蛋白,这种分辨率的提高对于归属和结构解析可能至关重要。