Siebeck M, Spannagl M, Hoffmann H, Schramm W, Fritz H
Department of Surgery, Klinikum Innenstadt, University of Munich, Germany.
Blood Coagul Fibrinolysis. 1991 Jun;2(3):459-64. doi: 10.1097/00001721-199106000-00009.
To investigate short-term activation and inhibition of fibrinolysis during shock, we studied plasma levels of tissue-type plasminogen activator (t-PA) and t-PA inhibition capacity (PAI) in anaesthetized pigs. t-PA in euglobulin fractions of plasma was measured by the conversion of plasminogen to plasmin in the presence of fibrin split products. Plasmin thus generated was measured in a chromogenic substrate assay. PAI was measured as plasma inhibition capacity for human melanoma t-PA. Controls (n = 8) had constant t-PA and PAI for 6 h. Lipopolysaccharide from Salmonella abortus equi in four different doses (n = 9 - 11), or live Escherichia coli (n = 3) induced a transient t-PA increase with peak values at 2 h. PAI decreased to 50% at 2 h and increased to 250% at 6 h. Phorbol myristate acetate (n = 7) induced no change of t-PA or PAI. Dextran sulphate (n = 4) produced a t-PA rise at 30 min, followed by a rapid decline. Endotoxin was an appropriate stimulus for activation and inhibition of fibrinolysis whereas phorbol ester failed to elicit this response.
为研究休克期间纤维蛋白溶解的短期激活和抑制情况，我们对麻醉猪的血浆组织型纤溶酶原激活物（t-PA）水平和t-PA抑制能力（PAI）进行了研究。血浆优球蛋白组分中的t-PA通过在纤维蛋白降解产物存在的情况下将纤溶酶原转化为纤溶酶来测定。如此产生的纤溶酶在显色底物测定中进行测量。PAI作为对人黑色素瘤t-PA的血浆抑制能力进行测量。对照组（n = 8）在6小时内t-PA和PAI保持恒定。四种不同剂量（n = 9 - 11）的马流产沙门氏菌脂多糖或活大肠杆菌（n = 3）诱导t-PA短暂升高，峰值出现在2小时。PAI在2小时时降至50%，在6小时时升至250%。佛波酯肉豆蔻酸酯（n = 7）未引起t-PA或PAI的变化。硫酸葡聚糖（n = 4）在30分钟时使t-PA升高，随后迅速下降。内毒素是激活和抑制纤维蛋白溶解的合适刺激物，而佛波酯未能引发这种反应。
