Canal-Raffin Mireille, Receveur Mathilde, Martinez Béatrice, Titier Karine, Ohayon Celine, Baldi Isabelle, Molimard Mathieu, Moore Nicholas, Brochard Patrick
Departement de Pharmacologie, INSERM U657, Université Victor Segalen Bordeaux 2, 33076 Bordeaux, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):106-13. doi: 10.1016/j.jchromb.2008.02.011. Epub 2008 Feb 23.
Solid-phase extractions followed by HPLC-UV/DAD methods were developed for occupational biological monitoring or forensic investigations of the fungicide folpet using its degradation products, phthalimide and phthalamic acid as plasma biomarkers. These methods show good linearity (r>0.9955), precision (CV<15%) and accuracy (bias<14.8%). The lower limits of quantification for phthalimide and phthalamic acid were 10 and 20 ng/ml and the absolute recoveries were higher than 86% and 68%, respectively. Applying these methods, a plasma toxicokinetic study of folpet in rats after intratracheal administration of Folpan 80WG showed that inhalation of folpet could be a route of exposure with an important systemic absorption.
开发了固相萃取结合高效液相色谱-紫外/二极管阵列检测法，用于职业生物监测或法医调查，以杀菌剂灭菌丹的降解产物邻苯二甲酰亚胺和邻苯二甲酸作为血浆生物标志物来检测杀菌剂灭菌丹。这些方法具有良好的线性（r>0.9955）、精密度（CV<15%）和准确度（偏差<14.8%）。邻苯二甲酰亚胺和邻苯二甲酸的定量下限分别为10和20 ng/ml，绝对回收率分别高于86%和68%。应用这些方法，对大鼠气管内给予Folpan 80WG后进行的灭菌丹血浆毒代动力学研究表明，吸入灭菌丹可能是一种具有重要全身吸收的暴露途径。
