Nguyen Anne Q N, Théorêt Yves, Chen Chunlin, Denault André, Varin France
Faculté de pharmacie de l'Université de Montréal, Canada.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Mar 1;877(7):657-60. doi: 10.1016/j.jchromb.2009.01.024. Epub 2009 Jan 24.
An improved analytical assay was developed and validated for the quantification of milrinone concentrations in patients undergoing cardiac surgery. A solid-phase extraction was optimized to isolate milrinone from a plasma matrix followed by HPLC using UV detection. Plasma samples (1 ml) were extracted using a C(18) solid-phase cartridge. Milrinone was separated on a strong cation exchange analytical column maintained at 23.4 degrees C. The mobile phase consisted of a gradient (10:90 to 45:55), 0.05 M phosphate buffer (pH 3):acetonitrile. Calibration curves were linear in the concentration range of 1.25-320 ng/ml. Mean drug recovery and accuracy were respectively > or =96% and > or =92%. Intra- and inter-day precisions (CV%) were < or =6.7% and < or =7.9%, respectively. This method proved to be reliable, specific and accurate. Using different types of column for extraction and separation of milrinone proved to be necessary to achieve the sensitivity and specificity required when milrinone is given by inhalation.
开发并验证了一种改进的分析方法，用于定量心脏手术患者体内的米力农浓度。优化了固相萃取方法，从血浆基质中分离米力农，随后采用紫外检测的高效液相色谱法。使用C(18)固相柱提取1 ml血浆样品。米力农在温度保持在23.4℃的强阳离子交换分析柱上分离。流动相为梯度洗脱（10:90至45:55），0.05 M磷酸盐缓冲液（pH 3）：乙腈。校准曲线在1.25 - 320 ng/ml浓度范围内呈线性。平均药物回收率和准确度分别≥96%和≥92%。日内和日间精密度（CV%）分别≤6.7%和≤7.9%。该方法被证明是可靠、特异且准确的。事实证明，使用不同类型的柱来萃取和分离米力农对于实现吸入米力农时所需的灵敏度和特异性是必要的。
