Boutrin M-C F, Foster H A, Pentreath V W
Centre for Parasitology and Infectious Diseases, Biomedical Sciences Research Institute, School of Environment and Life Sciences, University of Salford, The Crescent Salford, Lancs M5 4WT, United Kingdom.
Exp Parasitol. 2008 Jun;119(2):246-51. doi: 10.1016/j.exppara.2008.02.002. Epub 2008 Feb 9.
The potential role of phospholipases in trypanosomiasis was investigated using bee venom phospholipase A2 (bvPLA2) as a model. The effects of bvPLA2 on the survival of Trypanosoma brucei brucei, 2h and 12h cultures of Enterobacter cloacae, Escherichia coli, Citrobacter freundii were studied. About 1 mg ml(-1) bvPLA2 was trypanocidal after 30 min. Some growth occurred at lower concentrations up to 2h after treatment but viability decreased up to 8h. Even very low concentrations of bvPLA2 (10(-12) mg ml(-1)) had some trypanocidal activity. Bee venom PLA2 was bactericidal to 2h bacterial cultures but bacteriostatic to 12h ones. Minimum bactericidal concentrations were 10(-5)-10(-6) mg ml(-1). The results showed that bvPLA2 had significant trypanocidal and antibacterial effects on Gram-negative bacteria. The relationship to events occurring during infection is discussed. Phospholipases may play a role in increased endotoxin levels in trypanosomiasis.
以蜂毒磷脂酶A2（bvPLA2）为模型，研究了磷脂酶在锥虫病中的潜在作用。研究了bvPLA2对布氏布氏锥虫存活的影响，以及对阴沟肠杆菌、大肠杆菌、弗氏柠檬酸杆菌2小时和12小时培养物的影响。约1 mg/ml的bvPLA2在30分钟后具有杀锥虫作用。在较低浓度下，处理后2小时内会有一些生长，但直至8小时活力下降。即使是极低浓度的bvPLA2（10^(-12) mg/ml）也具有一定的杀锥虫活性。蜂毒PLA2对2小时的细菌培养物具有杀菌作用，但对12小时的培养物具有抑菌作用。最低杀菌浓度为10^(-5)-10^(-6) mg/ml。结果表明，bvPLA2对革兰氏阴性菌具有显著的杀锥虫和抗菌作用。文中讨论了其与感染过程中发生事件的关系。磷脂酶可能在锥虫病内毒素水平升高过程中发挥作用。
