Salvianti Francesca, Bettini Priscilla P, Giordani Edgardo, Sacchetti Patrizia, Bellini Elvio, Buiatti Marcello
Dipartimento di Biologia Animale e Genetica Leo Pardi, Università degli Studi di Firenze, via Romana 17-19, 50121 Firenze, Italy.
J Plant Physiol. 2008 Nov 28;165(17):1808-16. doi: 10.1016/j.jplph.2007.12.010. Epub 2008 Mar 17.
The molecular interaction between pear tree (Pyrus spp.) and the phloem-feeding psylla Cacopsylla pyri (Linnaeus) was investigated through the construction and characterization of cDNA subtracted libraries. Genes expressed upon insect infestation were identified in the susceptible pear cultivar Bartlett and in the resistant selection NY10355. In both interactions, genes involved in the plant defense response were induced, confirming the observed similarity between the response to pathogens and to insects with piercing/sucking mouthparts. However, the two expression profiles were found to be different, with more genes involved in the response to biotic and abiotic stress being activated in the resistant plant than in the susceptible one. Further characterization of the identified genes could lead to the development of molecular markers associated with tolerance/resistance to pear psylla.
通过构建和鉴定cDNA消减文库，研究了梨树（梨属物种）与取食韧皮部的梨木虱（林奈梨木虱）之间的分子相互作用。在易感梨品种巴特利特和抗性选系NY10355中鉴定了昆虫侵染后表达的基因。在这两种相互作用中，参与植物防御反应的基因均被诱导，证实了对病原体和具有刺吸式口器昆虫的反应之间观察到的相似性。然而，发现这两种表达谱不同，与易感植物相比，抗性植物中有更多参与生物和非生物胁迫反应的基因被激活。对已鉴定基因的进一步表征可能会导致开发与对梨木虱的耐受性/抗性相关的分子标记。
