CC16（一种肺分泌蛋白）在大鼠中毒性肺损伤模型中的变化动力学及决定因素
Kinetics and determinants of the changes of CC16, a lung secretory protein in a rat model of toxic lung injury.
作者信息
Hantson Philippe, Bernard Alfred, Hermans Cedric
机构信息
Cliniques St. Luc, Université Catholique de Louvain, Brussels, Belgium.
出版信息
Clin Toxicol (Phila). 2008 Mar;46(3):230-8. doi: 10.1080/15563650701449448.
PURPOSE
To investigate the kinetics and the determinants of the changes of the Clara cell 16 kDa protein (CC16) as a marker of lung injury following exposure to different toxicants.
METHODS
Rats were treated with 4-Ipomeanol (IPO), alpha-naphtylthiourea (ANTU), sodium chromate (Na(2)CrO(4)) or paraquat-(PQ). The changes of CC16, total protein, albumin, and cystatin-C were determined on BALF supernatan, and CC16, cystatin-C and creatinine levels were also determined in serum.
RESULTS
Bronchiolar insult due to IPO or ANTU resulted in an initial transient increase of serum CC16, parallel to that of albumin in BALF, and a subsequent reduction in both BALF and serum. A slight reduction of CC16 in BALF was already apparent one hour following treatment with PQ. In the serum, CC16 increased to 400% of basal value. With PQ and Na(2)CrO(4), the elevation of CC16 was mainly determined by the degree of renal impairment.
目的
研究克拉拉细胞16 kDa蛋白（CC16）作为接触不同毒物后肺损伤标志物的变化动力学及决定因素。
方法
用4-异亚丙基丙酮（IPO）、α-萘硫脲（ANTU）、铬酸钠（Na₂CrO₄）或百草枯（PQ）处理大鼠。测定支气管肺泡灌洗（BALF）上清液中CC16、总蛋白、白蛋白和胱抑素C的变化，同时测定血清中CC16、胱抑素C和肌酐水平。
结果
IPO或ANTU引起的细支气管损伤导致血清CC16最初短暂升高，与BALF中白蛋白的升高平行，随后BALF和血清中的CC16均降低。用PQ处理1小时后，BALF中CC16就已明显轻微降低。在血清中，CC16升高至基础值的400%。对于PQ和Na₂CrO₄，CC16的升高主要由肾功能损害程度决定。
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