Ji F, Casper D P, Brown P K, Spangler D A, Haydon K D, Pettigrew J E
University of Illinois, Department of Animal Sciences, Urbana 61801, USA.
J Anim Sci. 2008 Jul;86(7):1533-43. doi: 10.2527/jas.2007-0262. Epub 2008 Mar 14.
The objective of this experiment was to determine the effect of a beta-glucanase-protease enzyme blend product (EBP) on fecal digestibility (FD), apparent ileal digestibility (AID), standardized ileal digestibility, and digestibility in the hindgut of growing pigs. Twelve ileal-cannulated, growing barrows (38.2 +/- 0.5 kg) were housed in individual metabolism crates, blocked by previous feed intake into 3 groups with 4 pigs each, and randomly assigned to 1 of 4 treatments within a square (group) of 3 replications of 4 x 4 Latin square design. Treatments were basal diet (Basal), Basal + 0.05% of EBP (0.05% EBP), Basal + 0.10% of EBP (0.10% EBP), and hydrolyzed casein for measurement of endogenous amino acids. The Basal diet consisted of corn and soybean meal and was calculated to have 3.36 Mcal of ME/kg and 1.1% of total lysine, as-fed basis. Feed intake of each replicate of the Latin square during the first period was 85% of the minimum feed intake of the 4 pigs during the preliminary period and was equalized within each square. The feeding level was increased by 100 g/d in each subsequent period. Each of the experimental periods was 14 d, including 4 d of dietary adaptation, 5 d of fecal collection, 3 d of transition period, and 2 d of ileal collection. Ileal effluents were collected continuously for the same 12-h interval each day. Pigs fed the EBP demonstrated increased (P < 0.05) FD of DM, OM, energy, CP, nonfiber carbohydrate, total dietary fiber, insoluble dietary fiber, acid-hydrolyzed fat, ash, Ca, and P compared with pigs fed Basal. The AID of NDF and hemicellulose was increased (P < 0.05) by supplying the EBP either at 0.05 or 0.10% in the diets, but AID of DM and energy was not increased. The AID of acid-hydrolyzed fat tended to be greater (P = 0.051) for the pigs fed the EBP than for those fed Basal. Ileal digestibility of most amino acids was not affected by treatment, but the EBP reduced the apparent and standardized digestibility of methionine, alanine, and serine (P < 0.05). The difference between FD and AID of hemicellulose was lower (P < 0.05) for the pigs fed the EBP than for those fed Basal. These results demonstrated that the EBP fed to growing pigs improved the FD of DM, OM, energy, CP, nonfiber carbohydrate, total dietary fiber, acid-hydrolyzed fat, Ca, and P, and the AID of NDF and hemi-cellulose, but the standardized ileal digestibility of amino acids was not improved by supplying the EBP in corn-soybean meal-based diets of growing pigs.
本试验旨在测定β-葡聚糖酶-蛋白酶复合酶产品（EBP）对生长猪粪便消化率（FD）、回肠表观消化率（AID）、标准回肠消化率以及后肠消化率的影响。选用12头安装了回肠瘘管的生长公猪（体重38.2±0.5 kg），单栏饲养于代谢笼中，根据之前的采食量分为3组，每组4头猪，并在4×4拉丁方设计的3个重复的方阵（组）内随机分配到4种处理之一。处理组分别为基础日粮（基础组）、基础日粮+0.05% EBP（0.05% EBP组）、基础日粮+0.10% EBP（0.10% EBP组）以及用于测定内源氨基酸的水解酪蛋白。基础日粮由玉米和豆粕组成，按饲喂状态计算，代谢能为3.36 Mcal/kg，总赖氨酸含量为1.1%。拉丁方设计每个重复在第一个阶段的采食量为预试期4头猪中最小采食量的85%，且在每个方阵内保持一致。在随后的每个阶段，采食量每天增加100 g。每个试验期为14天，包括4天的日粮适应期、5天的粪便收集期、3天的过渡期以及2天的回肠收集期。每天在相同的12小时时间段内连续收集回肠流出物。与饲喂基础日粮的猪相比，饲喂EBP的猪的干物质、有机物、能量、粗蛋白、非纤维碳水化合物、总膳食纤维、不溶性膳食纤维、酸水解脂肪、灰分、钙和磷的粪便消化率显著提高（P<0.05）。在日粮中添加0.05%或0.10%的EBP可提高中性洗涤纤维和半纤维素的回肠表观消化率（P<0.05），但干物质和能量的回肠表观消化率未提高。饲喂EBP的猪的酸水解脂肪回肠表观消化率有高于饲喂基础日粮猪的趋势（P=0.051）。大多数氨基酸的回肠消化率不受处理的影响，但EBP降低了蛋氨酸、丙氨酸和丝氨酸的表观消化率和标准消化率（P<0.05）。饲喂EBP的猪的半纤维素粪便消化率与回肠表观消化率之差低于饲喂基础日粮的猪（P<0.05）。这些结果表明，给生长猪饲喂EBP可提高干物质、有机物、能量、粗蛋白、非纤维碳水化合物、总膳食纤维、酸水解脂肪、钙和磷的粪便消化率以及中性洗涤纤维和半纤维素的回肠表观消化率，但在以玉米-豆粕为基础的日粮中添加EBP并不能提高生长猪氨基酸的标准回肠消化率。
