Yang Yi, Shou Zhangfei, Zhang Ping, He Qiang, Xiao Huaying, Xu Yifang, Li Chunmei, Chen Jianghua
Kidney Disease Center, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Genet Med. 2008 Mar;10(3):187-92. doi: 10.1097/GIM.0b013e318163c343.
To determine whether the main mitochondrial DNA (mtDNA) haplogroups of the Han people have an impact on long-term clinical outcome.
We prospectively studied 181 individuals who were sequentially admitted to the intensive care unit. Demographic and clinical data were recorded along with clinical outcome over 180 days. Follow-up was completed for all study participants. We then determined the mtDNA haplogroups of the patients and 570 healthy, age-matched Han people from Zhejiang province, Southeast China, by analyzing sequences of hypervariable mtDNA segments and testing diagnostic polymorphisms in the mtDNA coding region with DNA probes.
The frequency of the main subhaplogroups of the Han population in the study cohort did not differ significantly from the control group. mtDNA haplogroup R, one of the three main mtDNA haplogroups of the Han people, was a strong independent predictor for the outcome of severe sepsis, conferring a 4.68-fold (95% CI 1.903-10.844, P = 0.001) increased chance of survival at 180 days compared with those without the haplogroup R.
In the Han population, mtDNA haplogroup R was a strong independent predictor for the outcome of severe sepsis, conferring an increased chance of long-term survival compared with individuals without the R haplogroup.
确定汉族人群主要线粒体DNA（mtDNA）单倍群是否对长期临床结局有影响。
我们对181例依次入住重症监护病房的患者进行了前瞻性研究。记录了人口统计学和临床数据以及180天内的临床结局。所有研究参与者均完成随访。然后，我们通过分析mtDNA高变区序列并使用DNA探针检测mtDNA编码区的诊断性多态性，确定了患者以及来自中国东南部浙江省的570名年龄匹配的健康汉族人的mtDNA单倍群。
研究队列中汉族主要亚单倍群的频率与对照组无显著差异。汉族三大主要mtDNA单倍群之一的mtDNA单倍群R是严重脓毒症结局的强有力独立预测因素，与没有单倍群R的患者相比，180天时存活几率增加了4.68倍（95%CI 1.903 - 10.844，P = 0.001）。
在汉族人群中，mtDNA单倍群R是严重脓毒症结局的强有力独立预测因素,与没有R单倍群的个体相比，长期存活几率增加。
