Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Asian J Androl. 2013 Sep;15(5):630-3. doi: 10.1038/aja.2013.83. Epub 2013 Aug 5.
In this study, we aimed to determine whether the main mitochondrial DNA (mtDNA) haplogroups of the Han people have an impact on spermatozoa motility. We recruited 312 men who were consecutively admitted to two affiliated hospitals of College of Medicine, Zhejiang University from May 2011 to April 2012 as part of fertility investigations. Semen and whole blood samples were collected from the men. We determined the mtDNA haplogroups by analysing the sequences of mtDNA hypervariable segment I and testing diagnostic polymorphisms in the mtDNA coding region with DNA probes. No significant differences were found in the clinical characteristics of the mtDNA haplogroup R and non-R (P>0.05). Our results suggest that mtDNA haplogroup R is a strong independent predictor of sperm motility in the Han population, conferring a 2.97-fold (95% confidence interval: 1.74-4.48, P<0.001) decreased chance of asthenozoospermia compared with those without haplogroup R.
在这项研究中，我们旨在确定汉族人群的主要线粒体 DNA（mtDNA）单倍群是否会影响精子的运动能力。我们招募了 312 名男性，他们是 2011 年 5 月至 2012 年 4 月间连续入住浙江大学医学院附属医院的生育调查参与者。我们从这些男性中采集了精液和全血样本。我们通过分析 mtDNA 高变区 I 的序列以及使用 DNA 探针检测 mtDNA 编码区中的诊断多态性来确定 mtDNA 单倍群。mtDNA 单倍群 R 和非-R 的临床特征无显著差异（P>0.05）。我们的结果表明，mtDNA 单倍群 R 是汉族人群精子运动能力的一个强有力的独立预测因子，与没有单倍群 R 的人群相比，其弱精症的发生几率降低了 2.97 倍（95%置信区间：1.74-4.48，P<0.001）。
