Noori-Daloii Mohammad Reza, Rashidi-Nezhad Ali, Izadi Pantea, Hossein-Nezhad Arash, Sobhani Maryam, Derakhshandeh-Peykar Pupak, Alimoghaddam Kamran, Ghavamzadeh Ardeshir
Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ann Transplant. 2007;12(4):5-10.
Certain cytokine genotypes are associated with acute graft versus host disease (aGVHD) after bone marrow transplantation (BMT). The present study aimed to determine existing association between TGF-beta1 codon 10 polymorphism and aGVHD after HLA-identical sibling BMT in the Iranian population.
MATERIAL/METHODS: In a retrospective case-control study, 168 subjects including 84 Iranian HLA-identical sibling BMT donor/recipient pairs were recruited. All of the patients were affected by hematological malignancies (AML=39, ALL=23 and CML=22). PCR-SSP method was performed to determine TGF-beta1 codon 10 T/C polymorphism genotypes.
The frequency of TGF-beta1 codon 10 TT, TC and CC genotypes among all subjects were 26.8%, 33.3% and 39.9% respectively. Recipients with the T allele developed aGVHD significantly less than those without the T allele (odds ratio =0.334, P=0.026).
Genetic background of TGF-beta1 may be involved as a protective factor in the development of aGVHD in HLA-matched sibling BMT in Iranian population. Moreover, this finding may indicate that the genetic markers in Iranians are, at least to some extent, linked to distinct genetic event from Japanese.
某些细胞因子基因型与骨髓移植（BMT）后的急性移植物抗宿主病（aGVHD）相关。本研究旨在确定伊朗人群中，HLA匹配的同胞BMT后，转化生长因子β1（TGF-β1）第10密码子多态性与aGVHD之间的现有关联。
材料/方法：在一项回顾性病例对照研究中，招募了168名受试者，包括84对伊朗HLA匹配的同胞BMT供体/受体。所有患者均患有血液系统恶性肿瘤（急性髓细胞白血病=39例，急性淋巴细胞白血病=23例，慢性粒细胞白血病=22例）。采用聚合酶链反应-序列特异性引物（PCR-SSP）方法确定TGF-β1第10密码子T/C多态性基因型。
所有受试者中，TGF-β1第10密码子TT、TC和CC基因型的频率分别为26.8%、33.3%和39.9%。携带T等位基因的受体发生aGVHD的比例显著低于未携带T等位基因的受体（优势比=0.334，P=0.026）。
在伊朗人群HLA匹配的同胞BMT中，TGF-β1的遗传背景可能作为保护因素参与aGVHD的发生。此外，这一发现可能表明，伊朗人的遗传标记至少在一定程度上与日本人的不同遗传事件相关。
