Stramer Brian, Winfield Mark, Shaw Tanya, Millard Thomas H, Woolner Sarah, Martin Paul
Department of Physiology, School of Medical Sciences, University of Bristol, UK.
EMBO Rep. 2008 May;9(5):465-71. doi: 10.1038/embor.2008.34. Epub 2008 Mar 14.
By using a microarray screen to compare gene responses after sterile laser wounding of wild-type and 'macrophageless' serpent mutant Drosophila embryos, we show the wound-induced programmes that are independent of a pathogenic response and distinguish which of the genes are macrophage dependent. The evolutionarily conserved nature of this response is highlighted by our finding that one such new inflammation-associated gene, growth arrest and DNA damage-inducible gene 45 (GADD45), is upregulated in both Drosophila and murine repair models. Comparison of unwounded wild-type and serpent mutant embryos also shows a portfolio of 'macrophage-specific' genes, which suggest analogous functions with vertebrate inflammatory cells. Besides identifying the various classes of wound- and macrophage-related genes, our data indicate that sterile injury per se, in the absence of pathogens, triggers induction of a 'pathogen response', which might prime the organism for what is likely to be an increased risk of infection.
通过使用微阵列筛选技术，比较野生型和“无巨噬细胞”的蛇突变体果蝇胚胎在无菌激光损伤后的基因反应，我们展示了与致病反应无关的伤口诱导程序，并区分了哪些基因是巨噬细胞依赖性的。我们发现，在果蝇和小鼠修复模型中，一个这样的新的炎症相关基因——生长停滞和DNA损伤诱导基因45（GADD45）——均被上调，这突出了这种反应在进化上的保守性。对未受伤的野生型和蛇突变体胚胎的比较还显示了一组“巨噬细胞特异性”基因，这表明它们与脊椎动物炎症细胞具有类似的功能。除了鉴定各类与伤口和巨噬细胞相关的基因外，我们的数据表明，在没有病原体的情况下，无菌损伤本身会触发“病原体反应”的诱导，这可能使生物体面临感染风险增加的可能性。
