Sanchez-Diaz Alberto, Marchesi Vanessa, Murray Stephen, Jones Richard, Pereira Gislene, Edmondson Ricky, Allen Terry, Labib Karim
Cancer Research U.K., Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK.
Nat Cell Biol. 2008 Apr;10(4):395-406. doi: 10.1038/ncb1701. Epub 2008 Mar 16.
By rapidly depleting each of the essential budding yeast proteins of unknown function, we identified a novel factor that we call Inn1, which associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. We show that Inn1 has a C2 domain at the amino terminus of the protein that is required for ingression of the plasma membrane, whereas the remainder of the protein recruits Inn1 to the actomyosin ring. The lethal effects of deleting the INN1 gene can be suppressed by artificial fusion of the C2 domain to other components of the actomyosin ring, restoring membrane ingression on contraction of the actomyosin ring. Our data indicate that recruitment of the C2 domain of Inn1 to the contractile actomyosin ring is crucial for ingression of the plasma membrane during cytokinesis in budding yeast.
通过快速耗尽芽殖酵母中每种功能未知的必需蛋白质，我们鉴定出一种新因子，我们将其称为Inn1，它在有丝分裂末期与收缩性肌动球蛋白环结合，是胞质分裂所必需的。我们发现Inn1在蛋白质的氨基末端有一个C2结构域，这是质膜内陷所必需的，而蛋白质的其余部分将Inn1招募到肌动球蛋白环。通过将C2结构域人工融合到肌动球蛋白环的其他组分上，可以抑制删除INN1基因的致死效应，在肌动球蛋白环收缩时恢复质膜内陷。我们的数据表明，在芽殖酵母胞质分裂过程中，将Inn1的C2结构域招募到收缩性肌动球蛋白环对于质膜内陷至关重要。
