Maynard Nathaniel D, Chen Jing, Stuart Rhona K, Fan Jian-Bing, Ren Bing
Ludwig Institute for Cancer Research, University of California, San Diego, 9500 Gilman Ave., La Jolla, California 92037, USA.
Nat Methods. 2008 Apr;5(4):307-9. doi: 10.1038/nmeth.1194. Epub 2008 Mar 16.
We describe a high-throughput method, named ChIP-SNP, for the identification of allele-specific protein-DNA interactions throughout the human genome. ChIP-SNP combines chromatin immunoprecipitation (ChIP) with whole-genome single nucleotide polymorphism (SNP) genotyping microarray analysis. We demonstrated that it can be used to accurately identify allele-specific binding of RNA polymerase II (RNAP) in the human fibroblast genome, uncovering imprinted genes and other allele-specific binding events. ChIP-SNP will facilitate the study of mechanisms of allele-specific gene expression.
我们描述了一种名为ChIP-SNP的高通量方法，用于在整个人类基因组中鉴定等位基因特异性的蛋白质-DNA相互作用。ChIP-SNP将染色质免疫沉淀（ChIP）与全基因组单核苷酸多态性（SNP）基因分型微阵列分析相结合。我们证明，它可用于准确鉴定人类成纤维细胞基因组中RNA聚合酶II（RNAP）的等位基因特异性结合，揭示印记基因和其他等位基因特异性结合事件。ChIP-SNP将促进对等位基因特异性基因表达机制的研究。
